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GenWay/TMB CORE+ Reagent/GWB-Q00248/100 ml
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4000-520-616
AdditionalInformation:
Name | TMBCORE+Reagent |
---|---|
RelatedProductNames | TMBCORE+Reagents,ELISASolutions,TMBCORE+ |
ProductFormat | ReadytouseTMBsolution-liquid |
Applications | ELISA |
ApplicationInfo | ELISA:Neat |
Format | ReadyToUse |
StABIlity | 48monthsfromdateofdespatch. |
ReconstitutionandStorage | Storeat+4oC.DONOTFREEZE. Thisproductisphotosensitiveandshouldbeprotectedfromlight. Avoidexposuretoheatandcontaminationwithmetalionsorperoxidase. StoreinbottlesmadeofHighDensityPolyethylene(HDPE). ShelfLife 48monthsfromdateofdespatch. |
Storage | Storeat+4oC.DONOTFREEZE. Thisproductisphotosensitiveandshouldbeprotectedfromlight. Avoidexposuretoheatandcontaminationwithmetalionsorperoxidase. StoreinbottlesmadeofHighDensityPolyethy |
LeadTime | Domestic:within2-3weeksdelivery International:2-3weeks |
IntendedUse | ResearchUseOnly |
:: | IntendedUse:ThisproductisahighperformanceTMB(3,3",5,5"-tetramethylbenzidine)solution,recommendedforuseinELISAasasubstrateforhorserADIshperoxidase(HRP). ThisproductcontainsTMB,substratebufferandhydrogenperoxideinasafe,readytousesolution.TheactivityofTMBhasbeenoptimisedtoenableincreasedsensitivity,minimalbackgroundandrapiddevelopment. ThisproductproducesadeepbluecolourduringtheenzymaticdegradationofH2O2byhorseradishPeroxidase.Thereactionmaybestoppedwith0.2Msulphuricacid,resultinginayellowcolourreadat450nm. TMBPLUS2hassimilarsensitivitytoTMBPLUS(GWB-Q00251orGWB-Q00252),butanincreasedshelflife. |
:: | InstructionsForUse: 1.Itisrecommendedthat100ulofGWB-Q00248TMBsubstrateisusedpermicrotiterwell.Pourthedesiredamountofsubstrateintoasealedcontainerandallowittoreachroomtermperatureinthedark. 2.Add100ulsubstratesolutionpermicrotiterwell. 3.Allowdevelopmentofthesubstratesolution.Timeofdevelopmentistypically5-30minutes.Forbestresults,theplateshouldbekeptinthedarkduringincubatione.g.wrappedintinfoil. 4.Forkineticassays,readabsorbanceat655nm(blue).Forendpointassays,addanequalvolumeof0.2Msulphuricacidandreadtheabsorbanceat450nm(yellow).Thisendpointsolutionisstableforuptoonehour. N.B.Ifreducedintensityisrequired,itisrecommendedthatthedevelopmenttimeisreducedortheantibody/conjugateisdilutedfurther.(DilutionofGWB-Q00248isnotrecommended). |