ProductDetails | |
---|---|
Synonyms | Lipopolysaccharide(LPS)fromE.colistrainJ5(Rc),R-type(rough/mutant)LPS |
ProductType | Chemical |
Properties | |
Source/HostChemicals | IsolatedandpurifiedfromE.colistrainJ5. |
PurityChemicals | ≥99.9%.NodetectableDNA,RNAandproteintraces. |
Formulation | Liquid.Colourlessclearaqueoussolution. |
Concentration | 1mg/mlstABIlisedinsterile,double-distilledwater(ddWater),withoutanyadditives. |
BIOLOGicalActivity | Optimalconcentrationisdependentuponcelltype,species,desiredactivationandanalysis:0.05-1.0μg/ml.DoesnotactivateanyTLRotherthanTLR4astestedupto50μg/mlinrelevantcellularsystems(macrophages). |
Declaration | ManufacturedbyInnaxon. |
OtherProductData | TLRpure™:•QualifiedPurity&Activity•HighpotencyTLR4-specificLigands•Ultrapure(nodetectableprotein,RNAandDNA)•TestedonTLR4KOmurinemacrophages•StandardisedAqueousSterileSolutions•Nopurificationorhazardoussolubilisation•Excellentlot-to-lotconsistency |
ShippingandHandling | |
Shipping | AMBIENT |
ShortTermStorage | +4°C |
LongTermStorage | +4°C |
HandlingAdvice | Donotfreeze. Toyielda100μg/ml(1,000-100x)stocksolutionadd100μlofLPSto900μlendotoxin-freeandsterileddWater(Cat.No.:IAX-900-002)or0.9%NaClSolution(Cat.No.:IAX-900-003)andmixwell. PreparedilutedLPSworkingsolutionsjustpriortouse,keepsterile.Ready-madesolutioniscellculture-grade. |
Use/Stability | Stableforatleast2yearsafterreceiptwhenstoredat+4°C. |
Documents | |
MSDS | DownloadPDF |
ProductSpecificationSheet | |
Datasheet | DownloadPDF |
ActivationofcellsbyLPSismediatedbytheToll-likereceptor4(TLR4).ForoptimalinteractionwithLPS,TLR4requiresassociationwithmyeloiddifferentiationprotein2(MD-2).AccordingtocurrentconsensusactivationofTLR4isprecededbythetransferofLPStomembrane-bound(m)orsoluble(s)CD14byLPS-bindingprotein(LBP).Re-formLPSandlipidA,butnotS-formLPS,arecapableofinducingTNF-αresponsesalsointheabsenceofCD14.LPS,synthesizedbymostwild-type(WT)Gram-negativebacteria(S-formLPS),consistsofthreeregions,theO-polysaccharidechain,whichismadeupofrepeatingoligosaccharideunits,thecoreoligosaccharideandthelipidA,whichharborstheendotoxicactivityoftheentiremolecule.R-formLPSsynthesizedbytheso-calledrough(R)mutantsofGram-negativebacterialackstheO-specificchain.FurThermore,thecore-oligosaccharidemaybepresentindifferentdegreesofcompletion,dependingontheclass(RatoRe)towhichthemutantbelongs.LPSareamphipathicmoleculeswhosehydrophobicitydecreaseswithincreasinglengthofthesugarpart.Baseduponthesedifferences,S-andR-formLPSshowmarkeddifferencesinthekineticsoftheirbloodclearanceandcellularuptakeaswellasintheabilitytoinduceoxidativeburstinhumangranulocytesandtoactivatethehostcomplementsystem.
- AnewmethodfortheextractionofRlipopolysaccharides:C.Galanos,etal.;Eur.J.Biochem.9,245(1969)
- Electrodialysisoflipopolysaccharidesandtheirconversiontouniformsaltforms:C.Galanos&O.Lüderitz;Eur.J.Biochem.54,603(1975)
- ImmunoblotanalysisoftheR-formlipopolysaccharidefromSalmonellaSforms:S.Schlecht,etal.;Zentralbl.Bakteriol.277,288(1992)
- IsolationandpurificationofR-formlipopolysaccharides:C.Galanos&O.Lüderitz;MethodsinCarbohydrateChem.9,11(1993)
- TheinfluenceoflowgrowthtemperatureontheamountoffreeRlipopolysaccharide,ontheexpressionofR-coredeterminantsandonO-chainlengthsinSalmonellaSforms:S.Schlecht&H.Mayer;Zentralbl.Bakteriol.280,448(1994)
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
通过解答,教会我,谢谢
(1)优级纯试剂 亦称保证试剂,为一级品,纯度高,杂质极少,主要用于精密分析和科学研究,常以GR表示。
(2)分析纯试剂 亦称分析试剂,为二级品,纯度略低于优级纯,杂质含量略高于优级纯,适用于重要分析和一般性研究工作,常以AR表示。
(3)化学纯试剂 为三级品,纯度较分析纯差,但高于实验试剂,适用于工厂、学校一般性的分析工作,常以CP表示。
(4)实验试剂 为四级品,纯度比化学纯差,但比工业品纯度高,主要用于一般化学实验,不能用于分析工作,常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定,化学试剂的不同等级分别用各种不同的颜色来标志,见表1。
表1 我国化学试剂的等级及标志
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
A、Na2CO3HClH2SO4NaNO3
B、K2CO3H2SO4HNO3BaCl2
C、HClHNO3AgNO3NaCl
D、NaOHFeCl3MgCl2BaCl2
暂无品牌问答