Highlights
- Rapid method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes, including from humans, birds, rats, mice, cattle, etc.
- Ultra-high density BashingBeads are fracture resistant and chemically inert.
- Zymo-Spin column and unique filtration technologies effectively removes PCR inhibitors from the DNA product.
Description
Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
---|---|
Elution Volume | ≥ 50 µl |
Equipment | Microcentrifuge, vortex, cell disruptor/pulverizer |
Processing Volume | ≤150 mg feces, ≤ 250 mg soil, ≤100 mg fungal/bacterial cells (wet weight) |
Purity | A260/A280 nm ≥1.8. |
Sample Source | Feces or soil |
Sample Storage | DNA stored at ≤ -20°C. |
Size Range | Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
Type | Total DNA |
Yield | ≤ 25 µg total DNA |
Q1: My lysate seems viscous. What is causing this to happen? How can I fix this?
A viscous sample can indicate incomplete sample lysis. Try using less of your sample and optimize bead beating conditions (duration, speed, time) to ensure samples are thoroughly lysed. After bead beating, pellet the cell debris before moving on. Adding more Genomic Lysis buffer to the lysate can help dilute and deproteinate the sample, making the sample less viscous and more suitable for DNA recovery.
Q2: Are there any tips in optimzing bead beating conditions?
We have validated our kits with both high-speed homgenizers and low-speed disruptors. We highly recommend users to optimize their bead beating conditions for their own instruments. We recommend using a 2 ml-tube adapter to ensure that the bead beating is efficent (do not use adapters made of foam). For high-speed homogenizers, we recommend a total of 5 mins bead beating (1 min interval at 6.5 m/s with 5 mins rest, repeat 5 times). For low-speed cell disruptors, we recommend 30 mins at max speed.
Q3: What is the purpose of Zymo-Spin III-HRC step?
Environmental samples often contain inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanins, etc. that affect downstream applications such as PCR. Once the DNA is eluted off the binding column, the DNA is then passed through the Zymo-Spin III-HRC to remove the PCR inhibitors, and the DNA is then ready for downstream applications. The Zymo-Spin III-HRC does not bind DNA, it simply removes the PCR inhibitors. The Zymo-Spin III-HRC can be purchased separately as the OneStep PCR Inhibitor Kit (D6030).
Q4: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?
Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM). However, if bead beating is optimized and lysis is efficient, the addition of BME is not necessary and can be omitted.
Q5: When can an RNase A treatment be implemented in the protocol?
No additional RNase A treatment is required when processing samples within kit capacity. The selective chemistry allows for binding of double stranded DNA to the column and for RNA to flow through.
Cat # | Name | Size | Price | |
---|---|---|---|---|
S6012-50 | ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) | 50 Tubes | $101.00 | |
C1078-50 | Zymo-Spin IICR Columns | 50 Pack | $55.00 | |
C1058-50 | Zymo-Spin III-HRC Filters | 50 Pack | $108.00 | |
C1057-50 | Zymo-Spin III-F Filters | 50 Pack | $59.00 | |
D6001-3-40 | BashingBead Buffer | 40 ml | $29.00 | |
D6035-1-30 | Prep Solution | 30 ml | $18.00 | |
D3004-5-15 | DNA Pre-Wash Buffer | 15 ml | $10.00 | |
D3004-1-100 | Genomic Lysis Buffer | 100 ml | $60.00 | |
D3004-2-50 | g-DNA Wash Buffer | 50 ml | $18.00 | |
D3004-4-10 | DNA Elution Buffer | 10 ml | $14.00 |
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就是蛋白质分子的小片断
是氨基酸形成的
一、首先你要明白肽是什么...........................肽是氨基酸通过酰胺键结合而成的东西.........
二、你要明白氨基酸是什么..........................氨基酸是构成蛋白质的基本单位,多种氨基酸结合为长肽链,几条长肽链再盘旋就形成了蛋白质......................
三、关于小分子肽、短肽、多肽、寡肽.......其实都是肽.......区别只是由多少个氨基酸构成而已............
所以,你的问题可以很粗暴地理解为“蛋白质对人体有没有副作用”.......
如果你营养足够的情况下,再补充这个,会导致营养过剩,从而加重身体代谢的负荷............类似就是这样子的了...........小分子肽,一般现在用于化妆品上比较多(一ye子.植物肽面膜就是这个).......小分子肽(可以简单理解为纳米胶原蛋白),这比蛋白质(也可以粗暴理解为胶原蛋白)更加容易吸收......而用在食品上,要视乎是何种小分子肽了.....大豆肽、花生肽、大米肽....不同的肽有不同的功效.......主要可以改善风味、改善吸收、增强胃肠道功能等等.......
求助各位前辈,我最近在合成的化合物水溶性很好,非常好,以至于可以随便溶解在水里,它的六氟磷酸盐也可以随意溶解在水里(大于50uM),细胞成像实验显示它根本进不去细胞,求问有没有啥方法包裹一下让它进去?我搜了一下文献,感觉多数是把脂溶性特别好的东西包裹一下弄进去的,也许是搜索姿势不对没找到我需要的答案,**点拨啊!!!
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