CustomerValidation
- •ToxicolApplPharmacol.2016Dec15;313:195-203.
- •HarvardMedicalSchoolLINCSLIBRARY
Description | SB216763ispotentandselectiveglycogensynthasekinase-3(GSK-3)inhibitor,withIC50valueof34.3nMforGSK-3αandGSK-3β,respectively. |
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IC50&Target | IC50:34.3nM(GSK-3α),34.3nM(GSK-3β)[5] |
InVitro | SB-216763(10-20µM)inducesβ-cateninmediated-transcriptioninadose-dependentmannerinHEK293cells.SB-216763(10,15and20µM)canmaintainmESCswithapluripotent-likemorphologyinlong-termculture.SB-216763(10µM)canmaintainJ1mESCsinapluripotentstateformorethanamonth[2].SB-216763inhibitsGSK-3withIC50of34nM[3].SB-216763isequallyeffectiveatinhibitinghumanGSK-3αandGSK-3β[5]. |
InVivo | SB216763(20mg/kg,i.v.)significantlyimprovesthesurvivalofBLM-treatedmice.MicerandomizedtoreceiveBLMplusSB216763showsanoteworthyreduction,comparedwithBLM-treatedmice.SB216763(20mg/kg,i.v.)reducesthemagnitudeofBLM-inducedalveolitis[1].SB216763(0.2mg/kg,i.v.)witheither17β-E100orGeni100reversestheceilingeffectbecausetheseagentssignificantlyreduceinfarctsizewhentherabbits"heartsaresubmittedto30-minCAO[4]. |
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Pleaserefertothesolubilityinformationtoselecttheappropriatesolvent. | ||||||||||||||||
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CellAssay [2] | SB-216763isdissolvedin0.1%DMSO. MESCsmaintainedwithLIFor10µMSB-216763formorethanamonthareresUSPendedat40,000cells/mLinLIF-freemESCmedium.EBsarepreparedbyahangingdropprocedure.Briefly,20µLdropscontainingmESCsarePipettedontheinsideofa10-cmPetridishlid.ThelidsareplacedontoPetridishescontaining10mLofHBSSandtheEBsareallowedtoformandgrowfor4daysintheincubator.After4days,15-20EBsaretransferredtoawellcontainingLIF-freemESCmediumina24-wellplate.Themediumisexchangedeverytwodaysandautonomouslybeatingcellaggregatesareobservedandcounted.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly. | ||||||||||||||||
AnimalAdministration [1] | SB216763isdissolvedinvehicle(25%dimethylsulfoxide,25%polyethyleneglycol,and50%saline). Miceareallocatedtofourgroups(n=12/group)asfollows:1)intratrachealsaline+vehicle(25%dimethylsulfoxide,25%polyethyleneglycol,and50%saline),2)intratrachealsaline+SB216763(20mg/kg)dissolvedinvehicle,3)intratrachealBLM(3U/kg)+vehicle,and4)intratrachealBLM+SB216763(20mg/kg)invehicle.Anothersetofexperimentstoassesscytokineexpressionbyreversetranscription-PCRisconductedinthemice(n=12/group)toreceive1)intratrachealsaline+vehicle,2)intratrachealBLM,and3)intratrachealBLM+SB216763.Toinducepulmonaryfibrosis,BLMisintratracheallyadministeredinmice(n=15/group)onday0.BLMandsaline-treatedmiceareadministeredwithSB216763dissolvedinvehicleorvehiclealoneintravenouslyatday0andthenintraperitoneallytwiceaweekuntilday28.MicearesacrificedbyCO2 inhalationondays2,7,and28.IntheterminaldeoxynucleotidyltransferasedUTPnick-endlabeling(TUNEL)experiments,thecohortsofmiceareasfollows:saline-treated(n=6),BLM-treated(n=6),andBLM+SB216763-treated(n=6).MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly. | ||||||||||||||||
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MolecularWeight | 371.22 | ||||||||||||
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Formula | C₁₉H₁₂Cl₂N₂O₂ | ||||||||||||
CASNo. | 280744-09-4 | ||||||||||||
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Shipping | RoomtemperatureincontinentalUS;mayvaryelsewhere | ||||||||||||
Solvent&Solubility | 10mMinDMSO SB216763isdissolvedinDMSOtomakestocksolutionsandthensuspendedinnormalsaline[6]. *"<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation="">1> | ||||||||||||
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Purity:97.00%
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westblot蛋白免疫印迹实验跑小分子蛋白(15kd)条带一直是波浪状,有人说是胶的问题,可同时跑了36kd蛋白,条带是直的,有人说是要恒流跑,电压不要太大,我的转膜条件是50v,50min,请教各位大神指点,万分感谢!!!
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求助各位前辈,我最近在合成的化合物水溶性很好,非常好,以至于可以随便溶解在水里,它的六氟磷酸盐也可以随意溶解在水里(大于50uM),细胞成像实验显示它根本进不去细胞,求问有没有啥方法包裹一下让它进去?我搜了一下文献,感觉多数是把脂溶性特别好的东西包裹一下弄进去的,也许是搜索姿势不对没找到我需要的答案,**点拨啊!!!
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有机的是有机化合物的简称,它指的是含碳化合物.
但是,有四大类常见物质一般不作为有机物处理:
1、碳的氧化物,如CO和CO2.
2、碳酸及其盐,如CaCO3.
3、金属碳化物,如CaC2.
4、拟卤素及其化合物,如(CN)2与KSCN.
水的化学式为H2O,它不含有碳元素,故不是有机物.
但若所描述的水不是化学意义的水,而是自然界存在的天然水,那么,水中会溶有一定量的有机物.
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