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Medchemexpress/G-418 disulfate(Synonyms: Geneticin sulfate; Antibiotic G-418 sulfate)/HY-17561/1g
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Medchemexpress/G-418 disulfate(Synonyms: Geneticin sulfate; Antibiotic G-418 sulfate)/HY-17561/1g
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G-418(disulfate)isanaminoglycosideantibioticsimilarinstructuretogentamicinB1,whichblockspolypeptidesynthesisbyinhibitingtheelongationstepinbothprokaryoticandeukaryoticcells.
Description

G-418(disulfate)isanaminoglycosideantibioticsimilarinstructuretogentamicinB1,whichblockspolypeptidesynthesisbyinhibitingtheelongationstepinbothprokaryoticandeukaryoticcells.

InVitro

G418isaninhibitorofmanypro-andeukaryotesatconcentrationsfrom1-300microgram/mL.ResistancetoG418conferringbytheneogenefromTn5encodinganaminoglycoside3"-phosphotransferase,APT3"IIiscommonlyusedinlaboratoryresearchtoselectgeneticallyengineeredcells[1].Ingeneralforbacteriaandalgaeconcentrationsof5mg/Lorlessareused,formammaliancellsconcentrationsofapproximately400mg/Lareusedforselectionand200mg/Lformaintenance.Resistantclonesselectioncouldrequirefrom1toupto3weeks[2].

InVivo

G418(40and80mg/kg)forthreeconsecutivedaysaresufficienttoeliminateallnontransfectedT.bruceibruceiparasitesfrominfectedmice[3].

References
  • [1].DaviesJ,etal.Anewselectiveagentforeukaryoticcloningvectors.AmJTropMedHyg.1980Sep;29(5Suppl):1089-92.

    [2].LiY,etal.InhibitoryeffectsofantisenseRNAofHAb18G/CD147oninvasionofhepatocellularcarcinomacellsinvitro.WorldJGastroenterol.2003Oct;9(10):2174-7.

    [3].MurphyNB,etal.UseofaninvivosystemtodeterminetheG418resistancephenotypeofbloodstream-formTrypanosomabruceibruceitransfectants.AntimicrobAgentsChemother.1993May;37(5):1167-70.

AnimalAdmiNISTration
[3]

TocharacterizethesensitivityofthetrypanosomepopulationstoG418invivo,bloodstreamformsofT.bruceibruceiGUTat3.1andT.bruceibruceiGUTat3.1/BBR3areexpandedseparatelyinsublethallyirrADIatedmice.Priortothefirstpeakofparasitemia,trypanosomesarecollected,andaliquotscontaining106trypanosomesareinoculatedintraperitoneallyintomice.Twenty-fourhoursfollowinginfection,themicearedividedintogroupsandtreatedwithG418atadoseof10,20,30,40,50,or80mg/kgofbodyweight(bw)byinoculatingintraperitoneally0.2mLofthedruginsterilewater.At24and48hfollowingthefirsttreatment,G418isadministeredtoanimalsineachgroupatthesamedoseasbefore,resultinginthreetreatmentspermouse.RepeateddrugtreatmentsarenecessarytoensurecompleteeliminationofnontransfectedGUTat3.1parasitesfromthemice.Micearethenmonitoreddaily,for33days,forthepresenceofparasitesbymicroscopicexaminationofwet-bloodfilms.Animalsfoundtobeparasitemicarerecordedandthenremovedfromtheexperiment.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.

References
  • [1].DaviesJ,etal.Anewselectiveagentforeukaryoticcloningvectors.AmJTropMedHyg.1980Sep;29(5Suppl):1089-92.

    [2].LiY,etal.InhibitoryeffectsofantisenseRNAofHAb18G/CD147oninvasionofhepatocellularcarcinomacellsinvitro.WorldJGastroenterol.2003Oct;9(10):2174-7.

    [3].MurphyNB,etal.UseofaninvivosystemtodeterminetheG418resistancephenotypeofbloodstream-formTrypanosomabruceibruceitransfectants.AntimicrobAgentsChemother.1993May;37(5):1167-70.

MolecularWeight

692.71

Formula

C₂₀H₄₄N₄O₁₈S₂

CASNo.

108321-42-2

Storage
Powder-20°C3years
 4°C2years
Insolvent-80°C6months
 -20°C1month
Shipping

RoomtemperatureincontinentalUS;mayvaryelsewhere

Solvent&Solubility

H2O:≥30mg/mL

*"<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation="">

Purity:>98.0%