Millipore/ECM300 | QuantiMatrix Human Fibronectin ELISA/ECM300/1 kit_夹心法ELISA_ELISA试剂盒_试剂盒_蚂蚁淘，【正品极速】生物医学科研用品轻松购|ebiomall

Millipore/ECM300 | QuantiMatrix Human Fibronectin ELISA/ECM300/1 kit

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Description
Catalogue Number	ECM300
Brand Family	Chemicon®
Trade Name	Quantimatrix Chemicon
Description	QuantiMatrix Human Fibronectin ELISA
Overview	The QuantiMatrix™ ELISA kit for the determination of soluble human fibronectin provides sensitivity down to 10-20 ng/mL as determined from culture supernatants and purified protein samples. The assay is in the format of a competitive inhibition ELISA. Human fibronectin is pre-coated on the wells with the exception of row H, which serves as a reference. Standards and samples are diluted and pre-incubated with polyclonal rabbit antibody to human fibronectin. The polyclonal antibody binds to fibronectin in the standard dilutions, and in the sample, if present. The mixture is then transferred to the human fibronectin-coated plate. Free rabbit anti-human fibronectin binds to the fibronectin on the plate. Goat anti-rabbit IgG HRP conjugate reacts with bound rabbit anti-fibronectin. When HRP substrate is added, a blue color develops. The reaction is stopped by the addition of an acid, changing the color to yellow. This color is quantitated using an ELISA reader; the intensity of the color is inversely proportional to the amount of fibronectin in the original sample. A standard curve is constructed and sample values are interpolated.
Background Information	The determination of levels of extracellular matrix molecules is becoming more important to understanding alterations in matrix function during development and tumOrigenesis. Questions of matrix gene regulation and function require a sensitive means of determining the levels of protein expression by cells. The QuantiMatrix™ ELISA kit for the determination of soluble human fibronectin provides sensitivity down to 10-20 ng/mL as determined from culture supernatants and purified protein samples. The assay is in the format of a competitive inhibition ELISA. Human fibronectin is pre-coated on the wells with the exception of row H, which serves as a reference. Standards and samples are diluted and pre-incubated with polyclonal rabbit antibody to human fibronectin. The polyclonal antibody binds to fibronectin in the standard dilutions, and in the sample, if present. The mixture is then transferred to the human fibronectin-coated plate. Free rabbit anti-human fibronectin binds to the fibronectin on the plate. Goat anti-rabbit IgG HRP conjugate reacts with bound rabbit anti-fibronectin. When HRP substrate is added, a blue color develops. The reaction is stopped by the addition of an acid, changing the color to yellow. This color is quantitated using an ELISA reader; the intensity of the color is inversely proportional to the amount of fibronectin in the original sample. A standard curve is constructed and sample values are interpolated.

Description

Catalogue Number

ECM300

Brand Family

Chemicon®

Trade Name

Quantimatrix
Chemicon

Description

QuantiMatrix Human Fibronectin ELISA

Overview

The QuantiMatrix™ ELISA kit for the determination of soluble human fibronectin provides sensitivity down to 10-20 ng/mL as determined from culture supernatants and purified protein samples.

The assay is in the format of a competitive inhibition ELISA. Human fibronectin is pre-coated on the wells with the exception of row H, which serves as a reference. Standards and samples are diluted and pre-incubated with

polyclonal rabbit antibody to human fibronectin. The polyclonal antibody binds to fibronectin in the standard dilutions, and in the sample, if present. The mixture is then transferred to the human fibronectin-coated plate. Free rabbit anti-human fibronectin binds to the fibronectin on the plate. Goat anti-rabbit IgG HRP conjugate reacts with bound rabbit anti-fibronectin. When HRP substrate is added, a blue color develops. The reaction is stopped by the addition of an acid, changing the color to yellow. This color is quantitated using an ELISA reader; the intensity of the color is inversely proportional to the amount of fibronectin in the original sample. A standard curve is constructed and sample values are interpolated.

Background Information

The determination of levels of extracellular matrix molecules is becoming more important to understanding alterations in matrix function during development and tumOrigenesis. Questions of matrix gene regulation and function require a sensitive means of determining the levels of protein expression by cells. The QuantiMatrix™ ELISA kit for the determination of soluble human fibronectin provides sensitivity down to 10-20 ng/mL as determined from culture supernatants and purified protein samples.

The assay is in the format of a competitive inhibition ELISA. Human fibronectin is pre-coated on the wells with the exception of row H, which serves as a reference. Standards and samples are diluted and pre-incubated with polyclonal rabbit antibody to human fibronectin. The polyclonal antibody binds to fibronectin in the standard dilutions, and in the sample, if present. The mixture is then transferred to the human fibronectin-coated plate. Free rabbit anti-human fibronectin binds to the fibronectin on the plate. Goat anti-rabbit IgG HRP conjugate reacts with bound rabbit anti-fibronectin. When HRP substrate is added, a blue color develops. The reaction is stopped by the addition of an acid, changing the color to yellow. This color is quantitated using an ELISA reader; the intensity of the color is inversely proportional to the amount of fibronectin in the original sample. A standard curve is constructed and sample values are interpolated.

Product Information
Components	QuantiMatrixTM Fibronectin Coated Strips (Part No. 60103) - 1 x 96-well strip plate Human Fibronectin Standard (Part No. 60109) - 1 lyophilized bottle; reconstitute according to the protocol on page 2 Sample Diluent (Part No. 60095) - 1 x 15 mL bottle of 1% BSA in PBS + 0.01% Thimerosal Goat anti-Rabbit IgG-HRP Conjugate (QM2) (Part No. 60110) - 1 bottle; ready to use Rabbit anti-Human Fibronectin (Part No. 60111) - 1 bottle pre-diluted polyclonal antibody; ready to use TMB/E Substrate (Part No. 60096) - 1 bottle; ready to use Stop Solution (Part No. 60193) - 1 bottle; (0.5M HCl solution) Wash Buffer Concentrate (Part No. 60245) - 1 x 100 mL bottle; provides final concentrations of 0.05% Tween-20 and 0.01% Thimerosal when added to 900 mL of deionized water as described in step 1 of protocol on page 2.
Detection method	Chromogenic

Product Information

Components

QuantiMatrixTM Fibronectin Coated Strips (Part No. 60103) - 1 x 96-well strip plate
Human Fibronectin Standard (Part No. 60109) - 1 lyophilized bottle; reconstitute according to the protocol on page 2
Sample Diluent (Part No. 60095) - 1 x 15 mL bottle of 1% BSA in PBS + 0.01% Thimerosal
Goat anti-Rabbit IgG-HRP Conjugate (QM2) (Part No. 60110) - 1 bottle; ready to use
Rabbit anti-Human Fibronectin (Part No. 60111) - 1 bottle pre-diluted polyclonal antibody; ready to use
TMB/E Substrate (Part No. 60096) - 1 bottle; ready to use
Stop Solution (Part No. 60193) - 1 bottle; (0.5M HCl solution)
Wash Buffer Concentrate (Part No. 60245) - 1 x 100 mL bottle; provides final concentrations of 0.05% Tween-20 and 0.01% Thimerosal when added to
900 mL of deionized water as described in step 1 of protocol on page 2.

Detection method

Chromogenic

Storage and Shipping Information
Storage Conditions	Store the kit and all components at 2-8°C. PRECAUTIONS: Human fibronectin was purified from human plasma that tested negative for HBsAg and HIV. However, all materials should be handled carefully and in accordance with good laboratory practices. The instructions provided have been designed to optimize performance. Deviations from the suggested procedure may result in sub-optimal performance of the kit and failure to produce accurate data. Do not mix or substitute reagents from different kit lots.

Storage and Shipping Information

Storage Conditions

Store the kit and all components at 2-8°C.

PRECAUTIONS:

Human fibronectin was purified from human plasma that tested negative for HBsAg and HIV. However, all materials should be handled carefully and in accordance with good laboratory practices.

The instructions provided have been designed to optimize performance. Deviations from the suggested procedure may result in sub-optimal performance of the kit and failure to produce accurate data.

Do not mix or substitute reagents from different kit lots.

Applications
Application	The QuantiMatrix ELISA kit for the determination of soluble human fibronectin provides sensitivity down to 10-20 ng/mL as determined from culture supernatants & purified protein samples.
Key Applications	ELISA

Applications

Application

The QuantiMatrix ELISA kit for the determination of soluble human fibronectin provides sensitivity down to 10-20 ng/mL as determined from culture supernatants & purified protein samples.

Key Applications

ELISA

BIOLOGical Information
Species Reactivity	Human
Analytes Available	Fibronectin
Entrez Gene Number	NM_002026.2 NM_054034.2 NM_212474.1 NM_212475.1 NM_212476.1 NM_212478.1 NM_212482.1
Entrez Gene Summary	This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
Gene Symbol	FN1 DKFZp686O13149 DKFZp686H0342 DKFZp686I1370 MSF FN CIG DKFZp686F10164 LETS FINC
UniProt Number	P02751
UniProt Summary	FUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity). SIZE: 2386 amino acids; 262607 Da SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1. SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. PTM: Sulfated. SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.

BIOLOGical Information

Species Reactivity

Human

Analytes Available

Fibronectin

Entrez Gene Number

Entrez Gene Summary

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.

Gene Symbol

FN1
DKFZp686O13149
DKFZp686H0342
DKFZp686I1370
MSF
FN
CIG
DKFZp686F10164
LETS
FINC

UniProt Number

P02751

UniProt Summary

FUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity).
SIZE: 2386 amino acids; 262607 Da
SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1.
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
PTM: Sulfated.
SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.

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Millipore/ECM300 | QuantiMatrix Human Fibronectin ELISA/ECM300/1 kit

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