Millipore/ECM300 | QuantiMatrix Human Fibronectin ELISA/ECM300/1 kit_夹心法ELISA_ELISA试剂盒_试剂盒_蚂蚁淘，【正品极速】生物医学科研用品轻松购|ebiomall

Millipore/ECM300 | QuantiMatrix Human Fibronectin ELISA/ECM300/1 kit

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Description
CatalogueNumber	ECM300
BrandFamily	Chemicon®
TradeName	Quantimatrix Chemicon
Description	QuantiMatrixHumanFibronectinELISA
Overview	TheQuantiMatrix™ELISAkitforthedeterminationofsolublehumanfibronectinprovidessensitivitydownto10-20ng/mLasdeterminedfromculturesupernatantsandpurifiedproteinsamples. TheassayisintheformatofacompetitiveinhibitionELISA.Humanfibronectinispre-coatedonthewellswiththeexceptionofrowH,whichservesasareference.Standardsandsamplesaredilutedandpre-incubatedwith polyclonalrabbitantibodytohumanfibronectin.Thepolyclonalantibodybindstofibronectininthestandarddilutions,andinthesample,ifpresent.Themixtureisthentransferredtothehumanfibronectin-coatedplate.Freerabbitanti-humanfibronectinbindstothefibronectinontheplate.Goatanti-rabbitIgGHRPconjugatereactswithboundrabbitanti-fibronectin.WhenHRPsubstrateisadded,abluecolordevelops.Thereactionisstoppedbytheadditionofanacid,changingthecolortoyellow.ThiscolorisquantitatedusinganELISAreader;theintensityofthecolorisinverselyproportionaltotheamountoffibronectinintheoriginalsample.Astandardcurveisconstructedandsamplevaluesareinterpolated.
BackgroundInformation	ThedeterminationoflevelsofextracellularmatrixmoleculesisbecomingmoreimportanttounderstandingalterationsinmatrixfunctionduringdevelopmentandtumOrigenesis.Questionsofmatrixgeneregulationandfunctionrequireasensitivemeansofdeterminingthelevelsofproteinexpressionbycells.TheQuantiMatrix™ELISAkitforthedeterminationofsolublehumanfibronectinprovidessensitivitydownto10-20ng/mLasdeterminedfromculturesupernatantsandpurifiedproteinsamples. TheassayisintheformatofacompetitiveinhibitionELISA.Humanfibronectinispre-coatedonthewellswiththeexceptionofrowH,whichservesasareference.Standardsandsamplesaredilutedandpre-incubatedwithpolyclonalrabbitantibodytohumanfibronectin.Thepolyclonalantibodybindstofibronectininthestandarddilutions,andinthesample,ifpresent.Themixtureisthentransferredtothehumanfibronectin-coatedplate.Freerabbitanti-humanfibronectinbindstothefibronectinontheplate.Goatanti-rabbitIgGHRPconjugatereactswithboundrabbitanti-fibronectin.WhenHRPsubstrateisadded,abluecolordevelops.Thereactionisstoppedbytheadditionofanacid,changingthecolortoyellow.ThiscolorisquantitatedusinganELISAreader;theintensityofthecolorisinverselyproportionaltotheamountoffibronectinintheoriginalsample.Astandardcurveisconstructedandsamplevaluesareinterpolated.

Description

CatalogueNumber

ECM300

BrandFamily

Chemicon®

TradeName

Quantimatrix
Chemicon

Description

QuantiMatrixHumanFibronectinELISA

Overview

TheQuantiMatrix™ELISAkitforthedeterminationofsolublehumanfibronectinprovidessensitivitydownto10-20ng/mLasdeterminedfromculturesupernatantsandpurifiedproteinsamples.

TheassayisintheformatofacompetitiveinhibitionELISA.Humanfibronectinispre-coatedonthewellswiththeexceptionofrowH,whichservesasareference.Standardsandsamplesaredilutedandpre-incubatedwith

polyclonalrabbitantibodytohumanfibronectin.Thepolyclonalantibodybindstofibronectininthestandarddilutions,andinthesample,ifpresent.Themixtureisthentransferredtothehumanfibronectin-coatedplate.Freerabbitanti-humanfibronectinbindstothefibronectinontheplate.Goatanti-rabbitIgGHRPconjugatereactswithboundrabbitanti-fibronectin.WhenHRPsubstrateisadded,abluecolordevelops.Thereactionisstoppedbytheadditionofanacid,changingthecolortoyellow.ThiscolorisquantitatedusinganELISAreader;theintensityofthecolorisinverselyproportionaltotheamountoffibronectinintheoriginalsample.Astandardcurveisconstructedandsamplevaluesareinterpolated.

BackgroundInformation

ThedeterminationoflevelsofextracellularmatrixmoleculesisbecomingmoreimportanttounderstandingalterationsinmatrixfunctionduringdevelopmentandtumOrigenesis.Questionsofmatrixgeneregulationandfunctionrequireasensitivemeansofdeterminingthelevelsofproteinexpressionbycells.TheQuantiMatrix™ELISAkitforthedeterminationofsolublehumanfibronectinprovidessensitivitydownto10-20ng/mLasdeterminedfromculturesupernatantsandpurifiedproteinsamples.

TheassayisintheformatofacompetitiveinhibitionELISA.Humanfibronectinispre-coatedonthewellswiththeexceptionofrowH,whichservesasareference.Standardsandsamplesaredilutedandpre-incubatedwithpolyclonalrabbitantibodytohumanfibronectin.Thepolyclonalantibodybindstofibronectininthestandarddilutions,andinthesample,ifpresent.Themixtureisthentransferredtothehumanfibronectin-coatedplate.Freerabbitanti-humanfibronectinbindstothefibronectinontheplate.Goatanti-rabbitIgGHRPconjugatereactswithboundrabbitanti-fibronectin.WhenHRPsubstrateisadded,abluecolordevelops.Thereactionisstoppedbytheadditionofanacid,changingthecolortoyellow.ThiscolorisquantitatedusinganELISAreader;theintensityofthecolorisinverselyproportionaltotheamountoffibronectinintheoriginalsample.Astandardcurveisconstructedandsamplevaluesareinterpolated.

ProductInformation
Components	QuantiMatrixTMFibronectinCoatedStrips(PartNo.60103)-1x96-wellstripplate HumanFibronectinStandard(PartNo.60109)-1lyophilizedbottle;reconstituteaccordingtotheprotocolonpage2 SampleDiluent(PartNo.60095)-1x15mLbottleof1%BSAinPBS+0.01%Thimerosal Goatanti-RabbitIgG-HRPConjugate(QM2)(PartNo.60110)-1bottle;readytouse Rabbitanti-HumanFibronectin(PartNo.60111)-1bottlepre-dilutedpolyclonalantibody;readytouse TMB/ESubstrate(PartNo.60096)-1bottle;readytouse StopSolution(PartNo.60193)-1bottle;(0.5MHClsolution) WashBufferConcentrate(PartNo.60245)-1x100mLbottle;providesfinalconcentrationsof0.05%Tween-20and0.01%Thimerosalwhenaddedto 900mLofdeionizedwaterasdescribedinstep1ofprotocolonpage2.
Detectionmethod	Chromogenic

ProductInformation

Components

QuantiMatrixTMFibronectinCoatedStrips(PartNo.60103)-1x96-wellstripplate
HumanFibronectinStandard(PartNo.60109)-1lyophilizedbottle;reconstituteaccordingtotheprotocolonpage2
SampleDiluent(PartNo.60095)-1x15mLbottleof1%BSAinPBS+0.01%Thimerosal
Goatanti-RabbitIgG-HRPConjugate(QM2)(PartNo.60110)-1bottle;readytouse
Rabbitanti-HumanFibronectin(PartNo.60111)-1bottlepre-dilutedpolyclonalantibody;readytouse
TMB/ESubstrate(PartNo.60096)-1bottle;readytouse
StopSolution(PartNo.60193)-1bottle;(0.5MHClsolution)
WashBufferConcentrate(PartNo.60245)-1x100mLbottle;providesfinalconcentrationsof0.05%Tween-20and0.01%Thimerosalwhenaddedto
900mLofdeionizedwaterasdescribedinstep1ofprotocolonpage2.

Detectionmethod

Chromogenic

StorageandShippingInformation
StorageConditions	Storethekitandallcomponentsat2-8°C. PRECAUTIONS: HumanfibronectinwaspurifiedfromhumanplasmathattestednegativeforHBsAgandHIV.However,allmaterialsshouldbehandledcarefullyandinaccordancewithgoodlaboratorypractices. Theinstructionsprovidedhavebeendesignedtooptimizeperformance.Deviationsfromthesuggestedproceduremayresultinsub-optimalperformanceofthekitandfailuretoproduceaccuratedata. Donotmixorsubstitutereagentsfromdifferentkitlots.

StorageandShippingInformation

StorageConditions

Storethekitandallcomponentsat2-8°C.

PRECAUTIONS:

HumanfibronectinwaspurifiedfromhumanplasmathattestednegativeforHBsAgandHIV.However,allmaterialsshouldbehandledcarefullyandinaccordancewithgoodlaboratorypractices.

Theinstructionsprovidedhavebeendesignedtooptimizeperformance.Deviationsfromthesuggestedproceduremayresultinsub-optimalperformanceofthekitandfailuretoproduceaccuratedata.

Donotmixorsubstitutereagentsfromdifferentkitlots.

Applications
Application	TheQuantiMatrixELISAkitforthedeterminationofsolublehumanfibronectinprovidessensitivitydownto10-20ng/mLasdeterminedfromculturesupernatants&purifiedproteinsamples.
KeyApplications	ELISA

Applications

Application

TheQuantiMatrixELISAkitforthedeterminationofsolublehumanfibronectinprovidessensitivitydownto10-20ng/mLasdeterminedfromculturesupernatants&purifiedproteinsamples.

KeyApplications

ELISA

BIOLOGicalInformation
SpeciesReactivity	Human
AnalytesAvailable	Fibronectin
EntrezGeneNumber	NM_002026.2 NM_054034.2 NM_212474.1 NM_212475.1 NM_212476.1 NM_212478.1 NM_212482.1
EntrezGeneSummary	Thisgeneencodesfibronectin,aglycoproteinpresentinasolubledimericforminplasma,andinadimericormultimericformatthecellsurfaceandinextracellularmatrix.Fibronectinisinvolvedincelladhesionandmigrationprocessesincludingembryogenesis,woundhealing,bloodcoagulation,hostdefense,andmetastasis.Thegenehasthreeregionssubjecttoalternativesplicing,withthepotentialtoproduce20differenttranscriptvariants.However,thefull-lengthnatureofsomevariantshasnotbeendetermined.
GeneSymbol	FN1 DKFZp686O13149 DKFZp686H0342 DKFZp686I1370 MSF FN CIG DKFZp686F10164 LETS FINC
UniProtNumber	P02751
UniProtSummary	FUNCTION:SwissProt:P02751#Fibronectinsbindcellsurfacesandvariouscompoundsincludingcollagen,fibrin,heparin,DNA,andactin.Fibronectinsareinvolvedincelladhesion,cellmotility,opsonization,woundhealing,andmaintenanceofcellshape.InteractionwithTNRmediatesinhibitionofcelladhesionandneuriteoutgrowth(Bysimilarity). SIZE:2386aminoacids;262607Da SUBUNIT:Mostlyheterodimersormultimersofalternativelysplicedvariants,connectedby2disulfidebondsnearthecarboxylends;toalesserextenthomodimers.InteractswithFBLN1,AMBP,TNR,LGALS3BPandCOL13A1. SUBCELLULARLOCATION:Secreted,extracellularspace,extracellularmatrix. TISSUESPECIFICITY:PlasmaFN(solubledimericform)issecretedbyhepatocytes.CellularFN(dimericorcross-linkedmultimericforms),madebyfibroblasts,epithelialandothercelltypes,isdepositedasfibrilsintheextracellularmatrix.Ugl-Y1,Ugl-Y2andUgl-Y3arefoundinurine.DEVELOPMENTALSTAGE:Ugl-Y1,Ugl-Y2andUgl-Y3arepresentintheurinefrom0to17yearsofage. PTM:Sulfated. SIMILARITY:SwissProt:P02751##Contains12fibronectintype-Idomains.&Contains2fibronectintype-IIdomains.&Contains16fibronectintype-IIIdomains.

BIOLOGicalInformation

SpeciesReactivity

Human

AnalytesAvailable

Fibronectin

EntrezGeneNumber

EntrezGeneSummary

Thisgeneencodesfibronectin,aglycoproteinpresentinasolubledimericforminplasma,andinadimericormultimericformatthecellsurfaceandinextracellularmatrix.Fibronectinisinvolvedincelladhesionandmigrationprocessesincludingembryogenesis,woundhealing,bloodcoagulation,hostdefense,andmetastasis.Thegenehasthreeregionssubjecttoalternativesplicing,withthepotentialtoproduce20differenttranscriptvariants.However,thefull-lengthnatureofsomevariantshasnotbeendetermined.

GeneSymbol

FN1
DKFZp686O13149
DKFZp686H0342
DKFZp686I1370
MSF
FN
CIG
DKFZp686F10164
LETS
FINC

UniProtNumber

P02751

UniProtSummary

FUNCTION:SwissProt:P02751#Fibronectinsbindcellsurfacesandvariouscompoundsincludingcollagen,fibrin,heparin,DNA,andactin.Fibronectinsareinvolvedincelladhesion,cellmotility,opsonization,woundhealing,andmaintenanceofcellshape.InteractionwithTNRmediatesinhibitionofcelladhesionandneuriteoutgrowth(Bysimilarity).
SIZE:2386aminoacids;262607Da
SUBUNIT:Mostlyheterodimersormultimersofalternativelysplicedvariants,connectedby2disulfidebondsnearthecarboxylends;toalesserextenthomodimers.InteractswithFBLN1,AMBP,TNR,LGALS3BPandCOL13A1.
SUBCELLULARLOCATION:Secreted,extracellularspace,extracellularmatrix.
TISSUESPECIFICITY:PlasmaFN(solubledimericform)issecretedbyhepatocytes.CellularFN(dimericorcross-linkedmultimericforms),madebyfibroblasts,epithelialandothercelltypes,isdepositedasfibrilsintheextracellularmatrix.Ugl-Y1,Ugl-Y2andUgl-Y3arefoundinurine.DEVELOPMENTALSTAGE:Ugl-Y1,Ugl-Y2andUgl-Y3arepresentintheurinefrom0to17yearsofage.
PTM:Sulfated.
SIMILARITY:SwissProt:P02751##Contains12fibronectintype-Idomains.&Contains2fibronectintype-IIdomains.&Contains16fibronectintype-IIIdomains.

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Millipore/ECM300 | QuantiMatrix Human Fibronectin ELISA/ECM300/1 kit

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