Description:
Description:ThePDE1BAssayKitisdesignedforidentificationofPDE1Binhibitorsusingfluorescencepolarization.TheassayisbasedonthebindingofafluorescentnucleotidemonophosphategeneratedbyPDE1Btothebindingagent.PDE1Bisacalcium-dependentcyclicnucleotidephosphodiesterasethatishighlyexpressedinthestriatum.Itplaysthephysiologicalroleinthecentralnervoussystem.ThePDE1Binhibitorscreeningassaykitcomesinaconvenient96-wellformat,withpurifiedPDE1Benzyme,fluorescentlylabeledPDE1Bsubstrate(cAMP),bindingagent,andPDEassaybufferfor100enzymereactions.ThekeytothePDE1BAssayKitisthespecificbindingagent.Usingthiskit,onlytwosimplestepsonamicrotiterplatearerequiredforPDE1Breactions.First,thefluorescentlylabeledcAMPisincubatedwithasamplecontainingPDE1Bfor1h.Second,bindingagentisaddedtothereactionmixtoproduceachangeinfluorescentpolarizationthatcanthenbemeasuredusingafluorescencereader.COMPONENTS:PDEassaybuffer:6mlFAM-Cyclic-3?,5"-AMP:50µl@20µMBindingagent:100µlBindingagentdiluent:10ul1blackplate200ngPDE1Bfor100reactionsAPPLICATIONS:GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.STORAGE:-20°Cor-70°C.REFERENCES:1.SiuciakJA,McCarthySA,ChapinDS,ReedTM,VorheesCV,RepaskeDR.Neuropharmacology2007;53(1):113-24.Format:assaykit
ScientificCategory:PDE
AdditionalInformation:
Name | PDE1BAssayKit |
---|---|
RelatedProductNames | PDE,phosphodiesterase,screening,inhibitors,PDE1B |
Format | AssayKit |
Storage | Mixedstorage |
IntendedUse | ResearchUseOnly |