Tissue collection, storage, microdissection, sectioning: See separate protocol.Tissue handling: Note that all fresh tissue should be handled as BioSafety Level 2 materials (wear gloves, lab coat, etc.).DNA extraction: The following protocol is based on a standard phenol DNA extraction protocol. Other protocols, and versions of this protocol, are also acceptable.1. Take pre-cut sampl......阅读全文
CELL CYCLE ANALYSIS
PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isol
E-Z 96® M13 Isolation Vacuum Manifold Protocol
实验概要The E.Z.N.A.™ family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids fr
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
Outline:Bacteriophage lambda is a linear double stranded DNA, approximately 50 kB in size. The two sticky ends help the phage to recircularize after e
293fectin™ Transfection
实验概要293fectin™ is a proprietary, cationic lipid-based formulation for transfection of DNA into eukaryotic cells. 293fectin™ is optimized f
Protein Expression and Purification Protocol
Step 1:Transformappropriate DNA plasmid into BL21(DE3)E. colicells. These cells must be competent. (Protocol for how to make c
DNA转化实验指导-1
CONTENTTransformation-CompetentE. colipreparationInoue ultra-competent methodRubidium chloride methodC
96-Well Sample Preparation for Suspension Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, T
Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation
实验概要Plasmid DNA is isolated from large-scale (500 ml) bacterial cultures by treatment with alkali and SDS.主要试剂Buffers and SolutionsAlkaline lysis solu
96-Well Sample Preparation for Suspension Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, T
Immunohistochemistry on paraffin embedded sections
主要试剂1. Neutral-buffered Formalin, 10% (NBF), 1 liter (Commercially available pre-prepared from many laboratory reagent suppliers).Double -distilled H
SYBR Green Quantitative PCR Protocol
SummaryQuantitative PCR is a method used to detect relative or absolute gene expression level. All qPCR involves the use of fluorescence to detect the
SSR GEL and Silver Staining Protocol
I.EQUIPMENT:DNA sequencing unit (35 x 45 cm) 2000V power supplyClampsLg. plastic trays (4), about 43 x 50 x 8 cm, and one lidTwo rocking p
Fast DNA 试剂盒及具体实验流程步骤
FastDNA试剂盒可从广阔的各种来源中快速有效地分离出 genomicDNA，配套 FastPrep仪器使用。动植物组织、细菌、海藻、真菌和其他许多标本，在 40秒内容易被裂解。这个 benchtop 设备是一种专利性的垂直角度运动，这种运动
Fungal Genomic DNA Extraction
实验概要This procedure does not require phenol extraction. The DNA is pure enough for restriction digests, PCR and genomic library construction.High t
A convenient method for the isolation of crude nuclear pellets.
This procedure describes a convenient method for the isolation of crude nuclear pellets fromN. crassa. The method, an adaptation of the one deve
Fibroblast Cell Systems-1
THESE INSTRUCTIONS APPLY TO ORDERS CONTAINING THE FOLLOWING CELL PRODUCTSCryopreserved Cells(Single donor)CC-2511NHDF -Ad3 500,000 cells/cryovia
罗氏诊断购买AvanSci Bio公司组织分离专利技术 美国时间2014年12月10日，罗氏诊断宣布与AvanSci Bio公司签署了一项收购协议，购买与高性能显微组织滑动式切片分离的相关产品，包括仪器、软件及研究人员使用的耗材，以及临床提取、具有较高精确度和纯度用于后续分子分析的特定区域，包括实时PCR、微阵列和测序。 AvanSci
ES Cell Culture and Manipulation
MediaHigh glucose DMEM (-pyruvate, -glutamine)20% Heat-inactivated Fetal calf serum (can vary by cell type, be sure!!)1X l-glutamine1X Penicillin/stre
Histopathological Approach to Rat Liver Tissue
ProcedureAfter deep ether anesthesia, dissect the rat’s liver (Wistar albino rats, 200 – 250 g) by cutting on the ventral side.Fix 2 – 3 mm. of the li
Fluorescence Procedures forthe ActinandTubulin Cytoskeleton inFixed Cells-2
Actin CytoskeletonMethanol fixationFix in -20oC methanol for 1-2.5 minutesRinse in TBSPermeabilize in TBS-0.5% TX for 10 minutesRinse in TBS-0.1% TX (
Fluorescence Procedures fortheActin andTubulin Cytoskeleton in Fixed Cells2
Formaldehyde FixationFix in 4% formaldehyde (16% stock EM grade) in CBS for 20 minutesRinse in TBSPermeabilize as for methanol fixationProcede as for
Establishment of Stable Transfectant of CHO Lec Cells
Purpose and BackgroundsCHO lec 3.2.8.1 cellsCHO Lec 3.2.8.1 cells have four independent mutations in the N- and O- glycosylation pathways (Stanley, 19
96-Well Sample Preparation for Adherent Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with adherent cells (HeLa, MCF-7, BALB
Real-time PCR
实验概要The exponential amplification via reverse transcription polymerase chain reaction provides for a highly sensitive technique in which a
Different types of human cells
Human primary cells are cells taken from living human beings and cultured. These cells retain the differentiation of the original cells take
96-Well Sample Preparation for Adherent Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with adherent cells (HeLa, MCF-7, BALB
96-Well Sample Preparation for Suspension Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, T
诱导性多能干细胞最新研究进展
诱导性多能干细胞（iPS细胞）最初是日本科学家山中伸弥（Shinya Yamanaka）团队在2006年利用病毒载体将四个转录因子（Oct4, Sox2, Klf4 和c-Myc）的组合转入到小鼠胚胎或皮肤纤维母细胞中，使其重编程而得到的类似胚胎干细胞的一种细胞类型。这些ips细胞在形态、基因和
三代测序的DNA提取和宏基因组学分析（二）
优势与局限这种DNA提取方法已被优化用于从人类粪便样本中提取高分子量(HMW)DNA，但也已在模拟微生物群落和细菌分离株上得到验证。我们希望该方案可以适用于其他样品类型，尽管可能需要对裂解前和裂解后步骤进行修改以解释特定于样品的制备和污染物清除的问题是必要的。机械裂解方法（如珠子击打）仍然是连续裂解
DNA测序
DNA测序（主要内容如下）·Sequencing Gel Preparation·P
