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...primordial germ cells defined by two monoclonal antibodies
2024-04-27
Gamete ResearchVolume 15, Issue 1 p. 25-34 Article Cell surface markers of mouse primordial germ cells defined by two monoclonal antibodies A. C. Hahnel, Corresponding Author Department of Biological Structure, University of Washington School of Medicine, SeattleDepartment of Medical Biochemistry, The University of Calgary, 3330 Hospital Drive N. W., Calgary, Alberta T2N 4N1 CanadaSearch for more papers by this authorE. M. Eddy, Department of Biological Structure, University of Washington School of Medicine, SeattleSearch for more papers by this author A. C. Hahnel, Corresponding Author Department of Biological Structure, University of Washington School of Medicine, SeattleDepartment of Medical Biochemistry, The University of Calgary, 3330 Hospital Drive N. W., Calgary, Alberta T2N 4N1 CanadaSearch for more papers by this authorE. M. Eddy, Department of Biological Structure, University of Washington School of Medicine, SeattleSearch for more papers by this author Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URLShare a linkShare onEmailFacebookTwitterLinked InRedditWechat Monoclonal antibodies EMA-1 and EMA-6 bind to cell surface antigens of mouse primordial germ cells between 7 and 14 days of development. Primordial germ cells can be identified by alkaline phosphatase activity, and EMA-1 and EMA-6 apparently recognize this same population of cells prior to 14 days of development. EMA-1 labels primordial germ cells in 8- to 13-day mouse embryos and EMA-6 in 8- to 10-day embryos. A difference in the cells recognized by EMA-1 and the lectin Dolichos biflorus agglutinin in early embryos suggests that these probes may be useful for studying primordial germ cells in situ or for isolating them from mouse embryos. The full text of this article hosted at iucr.org is unavailable due to technical difficulties. Please check your email for instructions on resetting your password. If you do not receive an email within 10 minutes, your email address may not be registered, and you may need to create a new Wiley Online Library account. Can\'t sign in? Forgot your username? Enter your email address below and we will send you your username If the address matches an existing account you will receive an email with instructions to retrieve your username
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