缓冲器 |鼻,Red Loading Buffer Pack

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缓冲器 |鼻,Red Loading Buffer Pack | NEB

2024-04-28

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Accept cookiesWe use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. To learn more and manage cookies, please refer to our Cookie Statement.Are you doing COVID-19 related research? Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes. For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplificationLearn about our tools that are helping researchers develop diagnostics and vaccines for the SARS-CoV-2 virus.Product Price ListDownload a PDF containing pricing for our full product list.Free ShippingSave time and money by placing an order with NEB. Take advantage of free shipping for any order totaling over $350. Place your order before 7:30pm EST for overnight delivery.For shipping questions click here.An alternative product is Blue Loading Buffer (NEB #B7703)The red loading buffer pack contains one vial of red loading buffer and one vial of 30X reducing agent. The combined solution is ideal for protein gel applications.1X Red Loading Buffer Composition: 187.5 mM Tris-HCl (pH 7.0), 6% SDS (w/v), 30% glycerol, 0.03% phenol red (w/v)30X Reducing Agent: 1.25 M DTTSDS interacts with positively charged amino acids of proteins, thereby disrupting interactions that form protein structures to separate proteins based on size rather than charge or shapeDTT is present to help reduce structural disulfide bonds Prepare fresh 3X Reducing Red Loading Buffer by adding 1/10 volume 30X Reducing Agent to 1 volume of 3X Red Loading Buffer. Prepare samples by adding 2 volumes of Sample to 1 volume 3X Reducing Red Loading Buffer (from step 1). Heat samples to 95-100 C for 3-5 minutes. After a quick microcentrifuge spin, load directly on to a gel. To ensure uniform mobiity, load an equal volume of 1X Reducing Blue Loading Buffer into any unused wells. Reagents stored at different temperatures. DTT will degrade at room temperature. Keep the two components separate at store at their proper temps. Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.For more information about commercial rights, please contact NEB\'s Global Business Development team at gbd@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.New England Biolabs (NEB) is committed to practicing ethical science we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.Ineligible item added to cartBased on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at freezers@neb.com or 1-800-632-5227 x 8.If you don t see your country above, please visit ourinternational siteSession ExpiredYou have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.Sign InYour profile has been mapped to an Institution, please sign back for your profile updates to be completed.Sign InTo save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.

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