Description:

Description: Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts. The Caspase-7 Inhibitor Drug Screening Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate DEVD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase-7 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase-7 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors. Summary: - Detection method- Fluorescence (Ex/Em 400/505)- Sample type- Cell samples treated with Caspase inhibitor drugs- Species reactivity- Mammalian - Kit size- 100 assays- Applications- Effective means for screening caspase inhibitors using fluorometric methods.Kit components: - 2X Reaction Buffer- Caspase Substrate DEVD-AFC (1 mM)- DTT (1 M)- Active Caspase-7 (Lyophilized)- Caspase Inhibitor, Z-VAD-FMK (2 mM)Features and Benefits: - Simple one-step procedure; takes only 1-1.5 hours- Fast and convenient- Inhibition of caspase-7 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors. Shipping Conditions: Gel pack

Additional Information:

Name Related Product Names Format Storage Intended Use

Caspase-7 Inhibitor Drug Screening Kit

Caspase-7 Inhibitor Drug Screening Kit, Caspase-7, Caspase 7 Caspase7, Caspase-7 Inhibitor, VAD, VAD-FMK, Z-VAD-FMK,Drug Screening Kit, caspase drug screening kit

Assay Kit

-20C

Research Use Only