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GenWay/PDE7B, Active Rat Recombinant Protein/GWB-2DC93C/10µg
2024-04-28
Description:
Description: Rat PDE7B (GenBank Accession No. NM_080894), a.a. 108-446(end), with N-terminal GST-tag, MW=65 kDa, expressed in a Baculovirus-infected Sf9 cell expression system. Specific Activity: 324 pmol/min/µg. Assay condition: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 200 µM cAMP, and 0.5-16 ng/μl rat PDE7B at 37°C for 20 min. Quantified by 5?-nucleotidase cleaving the 5-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Stability: >6 months at -80°C. References: 1. Gardner, C., et al. (2000). Biochem Biophys Res Commun. May 27;272(1):186-92. 2. Reyes-Irisarri, E., et al. (2005). Neuro-science 132(4):1173-85. 3. Zhang, L., et al. (2008). Proc. Natl. Acad. Sci. USA Dec 9;105(49):19532-7.Host: baculovirusTag: N-terminal GST tagGI Num: NM_080894MW: 65 kDaFormat: aqueous buffer solutionFormula: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathioneScientific Category: PDESpecies: ratAdditional Information:
| PDE7B, Active Rat Recombinant Protein |
| PDE, phosphodiesterase |
| 65 kDa |
| 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione |
| baculovirus |
| NM_080894 |
| Aqueous Buffer Solution |
| rat |
| -80°C |
| Research Use Only |
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