ARHGAP22 phospho S397 Antibody Properties Description Anti-ARHGAP22 pS397 (RABBIT) Antibody - 600-401-J12 Host Rabbit Target Species Mouse Known Cross Reactivity Human, Mouse, Rat Clonality Polyclonal Application ELISA : 1:20,000 - 1:60,000 IF Microscopy : 1:100-1:500 Western Blot : 1 µg/ml Immunohistochemistry: 1:100-1:500 Other Dilution: User Optimized Physical State Liquid (sterile filtered) Shipping Condition Dry Ice Format IgG Label Unconjugated Concentration 1.07 mg/ml by UV absorbance at 280 nm Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative None Stabilizer 50% (v/v) Glycerol with 1 mg/ml Bovine Serum Albumin (BSA) GeneName ARHGAP22 Relevant Links UniProtKB - Q8BL80NCBI - NP_722495GeneID - 239027

ARHGAP22 phospho S397 Antibody Description

Background ARHGAP22 is a Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. It acts as a GTPase activator for RAC1 by converting it to an inactive GDP-bound state and also inhibits RAC1-dependent lamellipodia formation. It may also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter. Anti-ARHGAP22 [p Ser397] antibody is ideal for researchers interested in Diabetes Research, Lipid and Metabolism research. Synonyms rabbit anti-ARHGAP pS397 antibody, ARHGAP 22, ARHGAP-22, Rho-type GTPase-activating protein 22, RHOGAP2, Rho GTPase activating protein 22, rho GTPase-activating protein 22 Immunogen ARHGAP22 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phospho-peptide corresponding to the region surrounding mouse pS397 region of ARHGAP22. Immunogen Type Peptide Post Translational Modification Phosphorylation Storage Condition Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Application Note Anti-ARHGAP22 pS397 antibody is useful for ELISA, Western Blot and Immunostaining. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~77.8 kDa corresponding to the appropriate cell lysate or extract. Purity/Specifity Anti-ARHGAP22 pS397 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for phosphorylated ARHGAP22 at Serine 397. It also recognizes the S22->A mutation but not the S397->A mutation. A BLAST analysis was used to suggest cross-reactivity with Mouse, Rat and Human based on 100% sequence homology. Cross-reactivity with ARHGAP22 pS397 from other sources has not been determined. Disclaimer Note-General This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.