Agrisera/Rabbit anti-Goat IgG (H&L), DyLight® 488 conjugated/AS09 623/
| | product information | | Background | | Rabbit anti-goat IgG (H&L), DyLight®488 Conjugated is a secondary antibody conjugated to DyLight® 488, which binds to Goat IgG (H&L) in immunological assays. DyLight®488 has Amax =493 nm, Emax =518 nm. Antibodies are purified using solid phase Goat IgG (H&L) DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries. | | Immunogen | | Purified Goat IgG, whole molecule | | Host | | Rabbit | | Clonality | | Polyclonal | | Clone | | | | Purity | | Affinity purified Rabbit IgG | | Format | | Lyophilized | | Quantity | | 1 mg | | Reconstitution | | For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily. | | Storage | | Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. | | Tested applications | | | | Related products | | other DyLight® anti-Goat secondary antibodies | | Additional information | | Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative. Based on immunoelectrophoresis, this antibody reacts with:, heavy (γ) chains on goat IgG, light chains on all goat immunoglobulinsNo reactivity is observed to: non-immunoglobulin goat serum proteins, IgG from human, mouse, or rat | |
| application information |
| Recommended dilution | | 1:20 - 1:2,000 for most applications |
| Expected | apparent MW | | |
| Confirmed reactivity | | |
| Predicted reactivity | | |
| Not reactive in | | |
| Additional information | | BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG. |
| Selected references | | to be added when available |
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