Agrisera/Rabbit anti-Mouse IgG (H&L), ALP conjugated, min. cross-reactivity to human serum/AS10 1235
| | product information | | Background | | Rabbit anti-mouse IgG (H&L), ALP (alkaline phosphatase) conjugated, is a secondary antibody which binds to mouse IgGs in immunological assays. Antibodies are adsorbed against human serum and affinity purified using solid phase mouse IgG. | | Host | | Rabbit | | Clonality | | Polyclonal | | Clone | | | | Purity | | Affinity purified IgG | | Format | | Liquid | | Quantity | | 1 mg | | Reconstitution | | | | Storage | | Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. | | Tested applications | | | | Related products | | other ALP conjugated secondary antibodies to mouse IgG | | Additional information | | This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin mouse or human serum proteins based on immunoelectrophoresis. Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative. | |
| application information |
| Recommended dilution | | 1:500-1:2000 for ELISA/western blot, 1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC). |
| Expected | apparent MW | | |
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