MCLAB/dCas9 3NLS/dCAS9-100/250 pmol, 50 µl-蚂蚁淘商城

新闻动态

MCLAB/dCas9 3NLS/dCAS9-100/250 pmol, 50 µl

2024-04-27

Cas9 Nuclease, Streptococcus pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA.

Introduction: Cas9 (CRISPR associated protein9) is a RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes.The CRISPR/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. Guided by a target-specific, single guide RNA (sgRNA), the Cas9 nuclease protein serves to unwind the genomic DNA duplex and cleave both strands upon recognition of the target sequence by the sgRNA, resulting in double-stranded breaks. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene.

$\"CRISPR-Cas9$

Description: Recombinant Cas9-NLS comprises the entire Cas9 protein sequence (1368 amino acids) fused to a proprietary nuclear localization sequence (NLS) and a 6 X His tag at the C-terminus. The protein has an apparent molecular weight of 162 kDa on SDS-PAGE.

Cas9 variants for various genome manipulations are available in 8 forms:

Cas9 (WT)
Cas9 with nuclear localization signal (NLS)
Cas9 with 3 NLS (on C-terminus) for better nuclear localization
NickaseCas9 (D10A) for nicking
dCas9 (D10A/H840A) for simple binding
Hi-Fi1 Cas9 3NLS (K848A, K1003A, R1060A) and Hi-Fi2 Cas9 3NLS (N497A, R661A, Q695A, Q926A) for reducing genome-wide off-target effects
dCas9-biotinylated for immunoprecipitation

Features: Low endotoxin Level High Biological activity Consistent from lot to lot

Application: Genome editing

Source: Cas9 gene from Streptococcus pyogenes expressed in E. coli

Purity: Greater than 97% as determined by SDS-PAGE.

Activity: 96% of PCR product digestion after 30 minutes at 37°C. The reaction is performed as follow; 20µl reaction, including 100nM Cas9 protein, 200nM tracrRNA, 200nM EMX1-specific crRNA, 200ng EMX1-specific PCR product and 1x digestion buffer (20mM HEPES, 100mM NaCl and 5mM MgCl2, pH 6.5). Incubate 30 minutes at 37°C, and stop reaction with 1µl RNAse A (1mg/ml).

Storage condition: -20°C

10X Cas9 Reaction Buffer: 200 mM HEPES, 50 mM MgCl2, 1 M NaCl, 1 mM EDTA, pH 6.5

Storage Buffer: 50 mM Tris-HCl 50 M KCl 1 mM DTT 1 mM EDTA 50% Glycerol pH 7.5

Experimental Data $\"Cas9-IMAGE-001.png\"$ $\"Cas9-IMAGE-002.png\"$

References

Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. (2012) A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. Aug 17;337 (6096):816-21.
Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. (2013) RNA-guided human genome engineering via Cas9. Science. Feb 15;339 (6121):823-6.

本文链接： https://www.ebiomall.com/b377-mclab/info-1483676849.html

免责声明本文仅代表作者个人观点，与本网无关。其创作性以及文中陈述文字和内容未经本站证实，对本文以及其中全部或者部分内容、文字的真实性、完整性、及时性本站不做任何保证或承诺，请读者仅作参考，并请自行核实相关内容。

没有了

MCLAB/dCas9 3NLS/dCAS9-100/250 pmol, 50 µl

2020-07-05

MCLAB/RNA-Seq Library Construction Kit/NGRR-200 /1 Ea

2020-07-15

MCLAB/LB Agar Blasticidin-50/LB-Bla502/1 Ea

2020-07-25

MCLAB/2YT Agar Chloramphenicol-34/2YT-Ach342/1 Ea

2020-08-07

MCLAB/CHAPS/DCPS101/1 Ea