MCLAB/MCMag PCR Purification Kit/MCPP-60/1 Ea
The MCMag PCR purification system utilizes solid-phase reversible immobilization (SPRI) paramagnetic bead technology for PCR amplicon purification.
Description The MCMag PCR purification system utilizes solid-phase reversible immobilization (SPRI) paramagnetic bead technology for PCR amplicon purification. The MCMag beads are pre-formulated with an optimized buffer to selectively bind DNA fragments of 100 bp and larger. Excess salts, enzymes, primers and nucleotides can be removed through a simple washing procedure. The MCMag PCR Purification system is fully adaptable to automation.Applications - PCR
- Sequencing
- Fragment Analysis
- Genotyping
- Cloning
- Primer Walking
Material Supplied - MCMag PCR Purification beads
- Store at 4°C upon arrival, for up to 18 months
- Shake the reagent well to a homogenous appearance before use
SpecificationsTable 1. Available product sizes | MCMag PCR Purification | Catalog Number | 5 mL | MCPP-5 | 60 mL | MCPP-60 | 450 mL | MCPP-450 |
Table 2. Number of PCR reactions purified with 96- and 384-well formats | Product Size | PCR reaction volume (µL) 96-well format | 5 mL | 60 mL | 450 mL | 10 | 278 rxns | 3332 rxns | 25000 rxns | 20 | 139 rxns | 1666 rxns | 12500 rxns | 50 | 56 rxns | 667 rxns | 5000 rxns | 100 | 28 rxns | 334 rxns | 2500 rxns | PCR reaction volume (µL) 384-well format | | | | 5 | 556 rxns | 6667 rxns | 50000 rxns | 7 | 397 rxns | 4762 rxns | 35714 rxns | 10 | 278 rxns | 3333 rxns | 20000 rxns | 14 | 198 rxns | 2381 rxns | 17857 rxns |
Materials Not Supplied - Reaction Plate
- McBead Magnetic Plate
- Plate Seals, Adhesive or Heat
- Wash Solution (70% ethanol)
- Elution Buffer
- Water
- Tris-Acetate (10 mM pH 8.0)
- TE Buffer (10 mM Tris-Acetate pH 8.0, 1 mM EDTA
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