首页 > AG Scientific > AG Scientific/HRV 3C Protease,Cysteine Protease, PreScission Site/H-1192/10 mg

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AG Scientific/HRV 3C Protease,Cysteine Protease, PreScission Site/H-1192/10 mg

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H-1192-10mg

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AG Scientific

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Humanrhinovirus3Cprotease(HRV3CProtease)isacysteineproteasethatrecognizesthecleavagesiteofLeu-Glu-Val-Leu-Phe-Gln-Gly-Pro,commonlyreferredtoasthePreScissionSite.ItcleavesbetweenGlnandGly. TherecombinantformoftheHRV3Cproteaseisarestrictiongradeproteasethathasrobustactivityat4°Candhighspecificactivity.ThisHRV3CProteaseisa47kDaproteinwithbothGSTandHistagssoitcanberemovedbyeitherNi-chelatingorGlutathione(GSH)resin.AmutationinthemouseAmelxtri-tyrosyldomainresultsinimpairedsecretionofamelogeninandphenocopieshumanX-linkedamelogenesis.ItisrecommendedtotesttheHRV3CProteasecleavagewithprotease-to-targetproteinratioof1:100(w/w)inthebuffersuitableforthetargetproteinat40°Covernight,withthetargetproteinconcentrationatorbelow1mg/ml.Underthiscondition,almost95%oftargetproteiniscleavedinmostcases.Theefficiencyofcleavagevariesduetothesequencesaroundthecleavagesite,theconformationandthesolubilityofthetargetprotein.IncludingreducingagentsuchasDTTorTCEPinthebufferhelpsthecleavage.Duetoitshighspecificity,moreproteasecanbeusedwithoutnon-specificcleavageoftargetproteins.Nonon-specificcleavageofcontrolcleavageproteinisobservedatprotease-to-controlcleavageproteinratioof1:10.AdditionalInformationSynonymsTurbo3C(HRV3C)ProteaseProduct#H-1192PreparationItisrecommendedtotesttheHRV3CProteasecleavagewithprotease-to-targetproteinratioof1:100(w/w)inthebuffersuitableforthetargetproteinat4°Covernight,withthetargetproteinconcentrationatorbelow1mg/ml.Underthiscondition,>9StorageTemp-20°CAdditionalInformationdecorateTable("product-attribute-specs-table")

AG Scientific, Inc.成立于1997年，专注于创新生化制剂以及用于诊断测试开发的定制原材料，以推进临床前研究药物开发。产品涉及抗生素，生物洗涤剂，缓冲液，诊断，酶，医疗设备试剂，蛋白酶K，还原剂，RNase A等。