Recombinant fragment within GST. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Run BLAST withRun BLAST withWB: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a GST-tagged Protein L-Myc (P12524; aa 1-394; 43kDa) construct. whole cell lysate.IP: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a GST-tagged Peroxiredoxin 5 (P30044; aa 1-214; 22kDa) construct whole cell lysate.
常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contactorders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:- High batch-to-batch consistency and reproducibility- Improved sensitivity and specificity- Long-term security of supply- Animal-free productionFor more information see here.
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液 pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 30% Glycerol (glycerin, glycerine)
The Abpromise guarantee
Abpromise™承诺保证使用ab252882于以下的经测试应用
\"应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
IP 1/30. WB 1/1000. Predicted molecular weight: 25 kDa. 说明 IP
1/30. WB
1/1000. Predicted molecular weight: 25 kDa. 靶标 相关性 GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. 别名 Glutathione S Transferase antibody Glutathione S-transferase class-mu 26 kDa isozyme antibody GST antibody Sj26 antigen antibody SjGST antibody see all 图片 Western blot - Anti-GST antibody [6G9] (ab252882)
All lanes : Anti-GST antibody [6G9] (ab252882) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty plasmid. whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a GST-tagged Protein L-Myc (P12524; aa 1-394; 43kDa) construct. whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/10000 dilution
Predicted band size: 25 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 59 seconds.
GST was immunoprecipitated from 0.35 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a GST-tagged Peroxiredoxin 5 (P30044; aa 1-214; 22kDa) construct whole cell lysate 10 g with ab252882 at 1/30 dilution (2 g in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252882 at 1/1000 dilution. Goat Anti-Rat IgG (H+L), HRP) (ab205720) was used at 1/5000 dilution.
Lane 1: HEK-293T transfected with a GST-tagged Peroxiredoxin 5 (P30044; aa 1-214; 22kDa) construct whole cell lysate 10 g.
Lane 2: ab252882 IP in HEK-293T transfected with a GST-tagged Peroxiredoxin 5 (P30044; aa 1-214; 22kDa) construct whole cell lysate.
Lane 3: Rat monoclonal IgG (ab18450) instead of ab252882 in HEK-293T transfected with a GST-tagged Peroxiredoxin 5 (P30044; aa 1-214; 22kDa)construct whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Click here to view the general protocols
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