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Summary:ImmunogenNB100-654=HumanHIF-1alphaaminoacids432-528.NB100-105=HumanHIF-1alphaaminoacids432-528.NB100-131=HumanHIF-1alphaaminoacids329-530.NB100-449=HumanHIF-1alphaaminoacids775-826.NB100-479=MouseHIF-1alphaaminoacidsSpecificityTheseantibodiesarespecificforHIF-1alpha.GeneHIF1AApplications/Dilutions:ApplicationNotesWesternBlot:AllantibodiesincludedinthispackwillworkforWesternanalysis,exceptforNB100-131,whichhasmixedresultsforWesternanalysis.Astartingdilutionof1:1,000isrecommendedwithtitrationupordowndependinguponthesignalandbackground.Immunohistochemistry:Bothpositiveandnegativefeedbackhavebeenprovidedonallantibodiesincludedinthispack.Astartingdilutionof1:250isrecommendedwithtitrationupordowndependinguponthesignalandbackground.ReviewedApplicationsRead2Reviewsrated4.5usingNB100-905inthefollowingapplication:WesternBlotPublicationsReadPublicationsusingNB100-905inthefollowingapplications:IHC1publicationIP1publicationWB2publicationsReactivityNotes:NB100-654recognizeshuman,mouseandrat.NB100-105recognizeshuman,sheep,mouse,rat,porcine,rabbit,bovineandferretHIF-1alpha.NB100-131recognizeshuman,mouseandratHIF-1alpha.NB100-449recognizeshumanandmouseHIF-1alpha.PrelimaryresultswithmousetissuehavebeenpositiveforIHCusingmouseeyesectionsat1:100withhighpHretrieval.NB100-479recognizeshumanandmouseHIF-1alpha.Packaging,Storage&Formulations:StorageStoreat4C.Donotfreeze.AlternateNamesfor53BP1Antibody:1vialeachof:NB100-654SS(0.025ml),NB100-479SS(0.025ml),NB100-449SS(0.025ml),NB100-105(0.05mL)andNB100-131(0.05mL).Background:ARNT-interactingproteinBasic-helix-loop-helix-PASproteinMOP1BHLHE78ClassEbasichelix-loop-helixprotein78HIF1AHIF1alphaHIF-1alphaHIF1AHIF-1aHIF-1-alphaHIF1-alphahypoxiainducIBLefactor1,alphasubunit(basichelix-loop-helixtranscriptionfactor)hypoxia-induciblefactor1-alphaMemberofPASprotein1memberofPASsuperfamily1MOP1HIF1-ALPHAPASdomain-containingprotein8PASD8alphasubunit(basichelix-loop-helixtranscriptionfactor)Limitations:1vialeachof:NB100-654SS(0.025ml),NB100-479SS(0.025ml),NB100-449SS(0.025ml),NB100-105(0.05mL)andNB100-131(0.05mL).
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