Summary:StandardCurveRange0.16-1ug/mlSensitivityTheminimumdetectabledoseofhumanHME-MMP12wasdeterminedtobeAssayTypeSandwichELISASampleVolume100uLKitTypeELISAKit(Colorimetric)GeneMMP12Applications/Dilutions:ApplicationNotesThehumanHME-MMP12ELISAkitisforquantitativedeterminationofHME-MMP12concentrationsinhumancellculturesupernatantsandBIOLOGicalfluids(plasma,serum,sputum).Thiskitisforresearchpurposeonly.PublicationsReadPublicationsusingDDXK-E-MMP12-1.Packaging,Storage&Formulations:ComponentsCaptureAntibody:0.5mg/mLofmouseanti-HME-MMP12monoclonalantibody(clone705D10)Standard:EachvialcontainsasolutionofrecombinantHME-MMP12(concentrationindicatedonthevial)produced,purifiedandconcentratedfromtransfectedeukaryoticcells.A8pointstandardcurveusing2-foldserialdilutionsinsampledilutionbufferDetectionAntibody:0.5mg/mLofHRPO-conjugatedanti-HME-MMP12mousemonoclonalantibody(clone706F9.01)Notes:ThisproductismanufacturedbyDendriticsanddistributedbyNovusBiologicalsinNorthAmericaandotherselectcountries.Toorderinthefollowingcountries:Germany,Finland,UnitedKingdom,Norway,Denmark,Switzerland,AustriaandSouthKorea,pleasecontactDendriticsforadditionalorderinginformation.AlternateNamesfor53BP1Antibody:EC3.4.24hMEHMEEC3.4.24.65Macrophageelastasemacrophagemetalloelastasematrixmetallopeptidase12(macrophageelastase)matrixmetalloproteinase12(macrophageelastase)Matrixmetalloproteinase-12MEMGC138506MMEMMP12MMP-12Background:Proteinsofthematrixmetalloproteinase(MMP)familyareinvolvedinthebreakdownofextracellularmatrixinnormalphysiologicalprocesses.HME/MMP-12,alsocalledmetalloelastase,iscriticalforinvasionanddestructioninpathologiessuchasaneurysmandemphysema.HMEmRNAandproteinwerepreviouslydescribedasexpressedinhumanalveolarmacrophages(CurciJAetal,1998,JClinInvest,102(11):1900-10).ThepredictedmolecularmassoftheHMEproenzymeis54kDaandreADIlyundergoesNH2-andCOOH-terminalprocessingtoanactive45KDaformandamature22kDaform.HMEisauniquehumanmetalloproteinasethatdisplayselastolyticactivity.WehaveisolatedHME-MMP12CDNAfromahumanCD34+-derivedDC(GM-CSF+TNF)library.ExpressionanalysisrevealedHME-MMP12expressioninlungandplacenta.Anti-HME-MMP12monoclonalantibodieswereobtainedaftermiceimmunizationwithHME-MMP12-transfectedeukaryoticcells.TheseantibodieswereusedtosetupaquantitativeimmunoassayforthedetectionofhumanHME-MMP12(DemedtsIKetal,2006;Thorax,61:196-201;Lapanetal,BMCPulmonaryMedicine,2010,10:40).Limitations:EC3.4.24hMEHMEEC3.4.24.65Macrophageelastasemacrophagemetalloelastasematrixmetallopeptidase12(macrophageelastase)matrixmetalloproteinase12(macrophageelastase)Matrixmetalloproteinase-12MEMGC138506MMEMMP12MMP-12

 

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