Summary:SpecificityThesecloneswereusedtosetupahumanMIP3-alphaspecificELISA,whichalsodetectsmacaqueMIP3alpha.Nocross-reactivitywithMIP1alpha,MIP1Beta,MIP1delta,MIP3beta,eotaxin,6Ckine,Rantes,MCP-1,TARC,MDC,TECK,SDF-1andIP-10wasdetected(Dieu-Nosjeanetal,J;Exp.med,2000192:705-18).StandardCurveRange0.01-20ng/mlSensitivityTheminimumdetectabledoseofhumanMIP3awasdeterminedtobeapproximately0.2ng/ml.AssayTypeSandwichELISASampleVolume100uLKitTypeELISAKit(Colorimetric)GeneCCL20Applications/Dilutions:ApplicationNotesThehumanMIP-3alphaELISAkitisforquantitativedeterminationofMIP3alphaconcentrationsinhumancellculturesupernatantsandBIOLOGicalfluids(plasma,serum).Thiskitisforresearchpurposeonly.PublicationsReadPublicationsusingDDXK-E-MIP-3-alpha.Packaging,Storage&Formulations:ComponentsDetectionAntibody:0.5mg/mLofHRPO-conjugatedanti-MIP3alphamousemonoclonalantibody(clone206D9).Standard:Eachvialcontains1g/mLofrecombinanthumanMIP3alphaproduced,purifiedandconcentratedfromeukaryoticcells.A7-pointstandardcurveusing2-foldserialdilutionsinsampledilutionbuffer,andahighstandardof20ng/mLisrecommendeCaptureAntibody:0.5mg/mLofmouseanti-MIP3alphamonoclonalantibody(clone319F6).Notes:ThisproductismanufacturedbyDendriticsanddistributedbyNovusBiologicalsinNorthAmericaandotherselectcountries.Toorderinthefollowingcountries:Germany,Finland,UnitedKingdom,Norway,Denmark,Switzerland,AustriaandSouthKorea,pleasecontactDendriticsforadditionalorderinginformation.AlternateNamesfor53BP1Antibody:betachemokineexodus-1Beta-chemokineexodus-1CCchemokineLARCC-Cmotifchemokine20CCL20chemokine(C-Cmotif)ligand20CKb4exodus-1LARCLARCLiverandactivation-regulatedchemokineMIP3alphaMIP-3alphaMIP-3aMIP-3-alphaMIP3AMacrophageinflammatoryprotein3alphaSCYA20Small-inducIBLecytokineA20smallinduciblecytokinesubfamilyA(Cys-Cys),member20ST38Background:Macrophageinflammatoryprotein(MIP-3a)/CCL20isaCC-typechemokinemappedtochromosome2inhumans.Langerhanscells(LCs)representauniquepopulationofDCscolonizingepithelium.MIP-3aplaysacentralroleinLCprecursorrecruitmentintotheepitheliumduringinflammation.(a)AmongDCpopulations,MIP-3aisthemostpotentchemokineinducingtheselectivemigrationofinvitro-generatedCD34+hematopoieticProgenitorcell-derivedLCprecursorsandskinLCs,inaccordancewiththerestrictedMIP-3areceptor(CCchemokinereceptor6)expressionbythesecells.(b)MIP-3aismainlyproducedbyepithelialcells,andthemigrationofLCprecursorsinducedbythesupernatantofactivatedskinkeratinocytesisblockedbyanantibodyagainstMIP-3a.(c)Invivo,MIP-3aisselectivelyproducedatsitesofinflammationasillustratedintonsilsandlesionalpsoriaticskinwhereMIP-3aupregulationisassociatedwithanincreaseinLCturnover.(d)ThesecretionofMIP-3aisstronglyupregulatedbycellsofepithelialoriginafterinflammatorystimuli(interleukin1bplusTNFa)orTcellsignals(HomeyB.etal,2000,J.Immunol.164:6621-32).Monoclonalantibodiesanti-MIP3awereobtainedaftermiceimmunizationwithMIP-3a-transfectedeukaryoticcells.ThesecloneswereusedtosetupahumanMIP-3aspecificElisa,whichalsodetectmacaqueMIP3a.Nocross-reactivitywithMIP-1a,MIP-1b,MIP-1d,MIP-3b,eotaxin,6Ckine,Rantes,MCP-1,TARC,MDC,TECK,SDF-1andIP-10wasdetected(Dieu-Nosjeanetal,J;Exp.med,2000192:705-18).Limitations:betachemokineexodus-1Beta-chemokineexodus-1CCchemokineLARCC-Cmotifchemokine20CCL20chemokine(C-Cmotif)ligand20CKb4exodus-1LARCLARCLiverandactivation-regulatedchemokineMIP3alphaMIP-3alphaMIP-3aMIP-3-alphaMIP3AMacrophageinflammatoryprotein3alphaSCYA20Small-induciblecytokineA20smallinduciblecytokinesubfamilyA(Cys-Cys),member20ST38

 

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