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Novus/Human TSLP ELISA Kit (Colorimetric) (DDXK-E-TSLP)/DDXK-E-TSLP/1 Kit
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Summary:StandardCurveRange0.31-20ng/mlSensitivityTheminimumdetectabledoseofhumanTSLPwasestimatedat1ng/mlAssayTypeSandwichELISASampleVolume100uLKitTypeELISAKit(Colorimetric)GeneTSLPApplications/Dilutions:ApplicationNotesThisisanenzyme-linkedimmunosorbentassayforquantitativedetection.Packaging,Storage&Formulations:ComponentsCaptureAntibody:0.5mg/mLofmouseanti-TSLPmonoclonalantibody(clone209A2.03).DiluteincarbonatebufferpH9.6toaworkingconcentrationof2.5ug/mLStandard:Eachvialcontains1ug/mLofrecombinantTSLPproduced,purifiedandconcentratedfromeukaryoticcells.A7-pointstandardcurveusing2-foldserialdilutionsinsampledilutionbuffer,andahighstandardof20ng/mLisrecommendedDetectionAntibody:0.5mg/mLofBiotin-conjugatedanti-TSLPmousemonoclonalantibody(clone210H1.04).Dilutetoaworkingconcentrationof3ug/mLinPBS-BSA1%-Tween0.05%.Notes:ThisproductismanufacturedbyDendriticsanddistributedbyNovusBIOLOGicalsinNorthAmericaandotherselectcountries.Toorderinthefollowingcountries:Germany,Finland,UnitedKingdom,Norway,Denmark,Switzerland,AustriaandSouthKorea,pleasecontactDendriticsforadditionalorderinginformation.AlternateNamesfor53BP1Antibody:thymicstromallymphopoietinTSLPBackground:TSLP(thymicstromallymphopoietin)isahemopoieticproteinof159aa(18kDa),producedmainlybynon-hematopoieticcellssuchasfibroblasts,epithelialcellsanddifferenttypesofstromalorstromallikecells.ItisknowntoplayanimportantroleinthematurationofTcellpopulationsthroughactivationofantigenpresentingcells.Itisalsodetectedinthethymusandthetissuecellsofthebonemarrow.TSLPaffectsthetransitionfrompre-BcellsinB.Alternativesplicingofthisgeneresultsintwotranscriptsvariants.TSLPisproposedtosignalthroughaheterodimericreceptorcomplexcomposedofTSLP-receptorandIL7Rchain.Expressedinmanytissues,itpreventsapoptosisandstimulatesthegrowthofmyeloidcells.(RechePAetal,JImmunol.,2001,167(1):336-43;Dorshkind.K,Nature.Immunol.,2000(1):369-370).MonoclonalantibodiesraisedagainsthumanTSLPwereobtainedaftermiceimmunizationwithTSLP-His-taggedtransfected293Tcells,andwereusedtosetupaquantitativeimmunoassaytodetermineTSLPconcentrationinbiologicalsamplesLimitations:thymicstromallymphopoietinTSLP
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