ImmunogenFunctions as a decoy by binding to the MyD88 TIR domain.PreparationMethodPreparation of 5 mM Stock SolutionsPBS* is added directly to the vials to prepare the stock solutions. Note: Bring the solution to room temperature and quick spin the tubes before opening the caps.MyD88 Homodimerization Inhibitor Peptide: 1 mg of DRQIKIWFQNRRMKWKKRDVLPGTAdd 64.4 ul of PBS* to the vial to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing. Control Peptide: 1 mg of DRQIKIWFQNRRMKWKKAdd 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing*Recipe for 1X PBS:1. Dissolve the following in 800ml distilled H2O.- 8g of NaCl- 0.2g of KCl- 1.44g of Na2HPO4- 0.24g of KH2PO4 2. Adjust pH to 7.5 with HCl.3. Adjust volume to 1L with additional distilled H2O. 4. Sterilize by autoclavingContent2 x 1.0mg: MyD88 Homodimerization Inhibitor peptide (lyophilized) DRQIKIWFQNRRMKWKKRDVLPGT (MyD88 homodimerization sequence: RDVLPGT). Molecular weight: 31002 x 1.0mg: Antennapedia Control peptide (lyophilized)DRQIKIWFQNRRMKWKK.Molecular weight: 2361 5mg size will contain 5mg of each peptide (MyD88 Homodimerization Inhibitor peptide and Antennapedia Control peptide). GeneMYD88DilutionsELISA 1:100 - 1:2000Application NotesInhibition of MyD88 dependent TLR/IL-1R signaling activitiy by interefering with MyD88 homodimer formation.Inhibitory peptide at 100 uM concentration may be a starting point. However, useful concentration of peptide may vary depending on experimental condition and cell type. Incubate cells for 24 hr with peptides before stimulating with ligands. Use in Functional inhibition reported in multiple pieces of scientific literature Use in In vivo assay reported in scientific literature (PMID 24755282)Use in blocking / neutralizing reported in scientific literature (PMID 24886588) Use in In vitro assay reported in scientific literature (PMID 25810567) Use in ELISA reported in scientific literature (PMID 23469306). Use in FLOW cytometry reported in scientific literature (PMID 27870876). Use in Binding inhibition reported in scientific literature (PMID 28302163). Use in Immunohistochemistryreported in scientific literature (PMID 28282921).Reviewed ApplicationsRead 6 Reviews rated 5 using NBP2-29328 in the following application:Immunocytochemistry/ImmunofluorescenceSerumPublicationsReadPublications using NBP2-29328 in the following applications: B/N 13 publications BindInhib 2 publications ELISA 2 publications FLOW 1 publication Func 2 publications Func-INH 2 publications Func-Inh 3 publications Human 1 publication IHC 3 publications In vitro 6 publications In vivo 4 publications InhibAct 2 publications WB 4 publications

 

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