Summary:ImmunogenHumanHIF-2alpha,correspondingtoaminoacids535-631.[UniProt#Q99814]SpecificityThisisspecificforHIF-2alphaanddoesnotcross-reactwithHIF-1alpha.IsotypeIgG1ClonalityMonoclonalHostMouseGeneEPAS1PurityProteinGpurifiedInnovator"sRewardTestinaspecies/applicationnotlistedabovetoreceiveafullcredittowardsafuturepurchase.LearnabouttheInnovator"sRewardApplications/Dilutions:DilutionsWesternBlot1-2ug/mlSimpleWestern1:100ChromatinImmunoprecipitationELISA1:100-1:2000FlowCytometry1:400Immunocytochemistry/ImmunofluorescenceImmunohistochemistry1:150-1:300Immunohistochemistry-Paraffin1:150-1:300ImmunoprecipitationGelSupershiftAssayApplicationNotesThisHIF-2alpha(ep190b)antibodyisusefulforELISA,FlowCytometry,Immunohistochemistryonparaffin-embeddedsectionsandWesternBlot.InWB,itrecognizesabandatapprox.118kDarepresentingHIF-2alpha.UseinGelsuppershiftassayreportedinscientificliterature(PMID17404621)InSimpleWesternonly10-15uLoftherecommendeddilutionisusedperdatapoint.SeparatedbySize-Wes,SallySue/PeggySue.Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.TheoreticalMW118kDa.Disclaimernote:Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.PositiveControlKidneyLysate(NB820-59231)HIF-2alpha/EPAS1Lysate(NBL1-10286)ReviewedApplicationsRead7Reviewsrated4.3usingNB100-132inthefollowingapplications:ImmunofluorescenceWesternBlotPublicationsReadPublicationsusingNB100-132inthefollowingapplications:ChIP8publicationsELISA1publicationEMSA1publicationICC/IF7publicationsIHC13publicationsIHC-Fr1publicationIHC-P14publicationsIP3publicationsWB71publicationsReactivityNotes:ABIlitytouseinmouseismixedwithsomepositiveandsomenegativeresults.Packaging,Storage&Formulations:StorageAliquotandstoreat-20Cor-80C.Avoidfreeze-thawcycles.BufferPBSwith1%BSAPreservative0.05%SodiumAzideConcentration1.0mg/mlPurityProteinGpurifiedNotes:Basic-helix-loop-helix-PASproteinMOP2BHLHE73bHLHe73ECYT4ClassEbasichelix-loop-helixprotein73endothelialPASdomainprotein1endothelialPASdomain-containingprotein1ep190bEPAS1EPAS-1HIF2AHIF-1alpha-likefactorHIF2alphaHIF-2alphahif2aangiogenesisHIF2AHIF2AHIF-2-alphaHLFHLFHIF-1-alpha-likefactorhypoxia-inducIBLefactor2alphaHypoxia-induciblefactor2-alphaMemberofPASprotein2MOP2MOP2HIF2-alphaPASD2PASdomain-containingprotein2AlternateNamesfor53BP1Antibody:Hypoxia-induciblefactors(HIFs)generatesheterodimerscomposedofalpha-subunit(HIF-1alpha,HIF-2alphaandHIF-3alpha)andbetasubunit(Arnt1,Arnt2andArnt3).Oftheseisoforms,HIF-1alphaandHIF-2alphaplayacriticalroleinthecellularresponsetohypoxicconditions.AfterheterodimerizationwithbetasubunitsandbindingtoDNAsequence5"-[AG]CGTG-3"withinHRE(hypoxiaresponseelement)oftargetgenepromoters,HIF-2alpharegulatesthegeneexpressioninresponsetooxygenhomeostasisalterations.HIF-2alpharegulatesVEGFexpressionforinvolvinginthedevelopmentofnewbloodvesselsaswellasthetubularsystemoflung.HIF-2alphaplayaroleintheformationoftheendotheliumthatgivesrisetobloodbrainbarrier.HIF-2alphaisastrongactivatorofexpressionofTie-2tyrosinekinasewhichisamajorplayerinangiogenesis.HIF-2alphaactivationrequirerecruitmentoftranscriptionalcoactivatorssuchasCREBPB,EP300etc.anditsinteractionwithredoxregulatoryproteinAPEXmediatesactivationofCTAD.HIF-2alpha/HIF-1alphaexpressionincreasesincertainmalignancies,wherein,HIF-1alphaplaysbothpro-aswellasanti-proliferativeactivities,whereas,HIF-2alphaissuggestedtoexertanti-proliferativeactivityonly.Background:Thisproductisforresearchuseonlyandisnotapprovedforuseinhumansorinclinicaldiagnosis.PrimaryAntibodiesareguaranteedfor1yearfromdateofreceipt.Limitations:Hypoxia-induciblefactors(HIFs)generatesheterodimerscomposedofalpha-subunit(HIF-1alpha,HIF-2alphaandHIF-3alpha)andbetasubunit(Arnt1,Arnt2andArnt3).Oftheseisoforms,HIF-1alphaandHIF-2alphaplayacriticalroleinthecellularresponsetohypoxicconditions.AfterheterodimerizationwithbetasubunitsandbindingtoDNAsequence5"-[AG]CGTG-3"withinHRE(hypoxiaresponseelement)oftargetgenepromoters,HIF-2alpharegulatesthegeneexpressioninresponsetooxygenhomeostasisalterations.HIF-2alpharegulatesVEGFexpressionforinvolvinginthedevelopmentofnewbloodvesselsaswellasthetubularsystemoflung.HIF-2alphaplayaroleintheformationoftheendotheliumthatgivesrisetobloodbrainbarrier.HIF-2alphaisastrongactivatorofexpressionofTie-2tyrosinekinasewhichisamajorplayerinangiogenesis.HIF-2alphaactivationrequirerecruitmentoftranscriptionalcoactivatorssuchasCREBPB,EP300etc.anditsinteractionwithredoxregulatoryproteinAPEXmediatesactivationofCTAD.HIF-2alpha/HIF-1alphaexpressionincreasesincertainmalignancies,wherein,HIF-1alphaplaysbothpro-aswellasanti-proliferativeactivities,whereas,HIF-2alphaissuggestedtoexertanti-proliferativeactivityonly.

 

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