首页 > Usbio > Usbio/376895 Deoxynivalenol (DON) ELISA BioAssay™ Kit/376895/48Tests

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Usbio/376895 Deoxynivalenol (DON) ELISA BioAssay™ Kit/376895/48Tests

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Deoxynivalenol(DON),alsoknownasVomitoxin,belongstotrichotheceneandisproducedbymouldfusarium,growingingrainssuchaswheat,maize,barleyandstover.Becauseofcausingvomittinginporcines,hencethenameisVomitoxin.IthassomeharmfuleffectstohumanbodyandhasbeenlistedasCategoryIIIcarcinogenbyEUclassificationcriteria.In2011,thelimitofDeoxynivalenolresiduesis1000ug/kgingrainsandgrainsproductsinChina.ThisBioAssay™kitisadetectionproductdevelopedbasedonELISAtechnology,withoperationtimeasshortas15minutes.SampleTypeFeed,soybeanmealIntendedUseThisBioAssay™kitallowsfortheinvitroquantitativedeterminationofDeoxynivalenolconcentrationsinfeed,soybeanmeal.Sensitivity1ug/kgRange1-81ug/kgPrecisionIntra-assay:CV%<10%Inter-assay:CV%<10%TestPrincipleThisBioAssay™employsthecompetitiveinhibitionenzymeimmunoassaytechnique.ThemicrotiterplateprovidedinthisBioAssay™kithasbeenpre-coatedwithDeoxynivalenolantigen.StandardsorsamplesareaddedtotheappropriatemicrotiterplatewellswithDeoxynivalenolantibodyandHorserADIshPeroxidase(HRP)conjugatedanti-antibody.Thecompetitiveinhibitionreactionislaunchedbetweenpre-coatedDeoxynivalenolandDeoxynivalenolinstandardsorsampleswiththeDeoxynivalenolantibody.AsubstratesolutionisaddedtothewellsandthecolordevelopsinoppositetotheamountofDeoxynivalenolinthestandardsorsamples.Thecolordevelopmentisstoppedandtheintensityofthecolorismeasured.KitComponentsAssayplate,96T/48TStandard(96T),6x1mlStandard(48T),6x0.5mlHRP-conjugate(96T),1x7mlHRP-conjugate(48T),1x3.5mlAntibody(96T),1x7mlAntibody(48T),1x1x3.5mlTMBSubstrate(96T),1x12mlTMBSubstrate(48T),1x6mlStopSolution(96T),1x10mlStopSolution,(48T),1x5mlSampleDiluent(96T),1x50mlSampleDIluent(48T),1x25mlWashBuffer(10X)(96T),1x50mlWashBuffer(10X)(48T),1x25mlAdhesiveStrip(96T/48T),4xstripsInstructionManual(96T/48T),1xmanualStorageandStABIlityStorepowderat4°Cliquidat-20°C.Storeothercomponentsat4°C.Stableforatleast6monthsFormaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.

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