Sample TypeSerum, Plasma, Biological FluidsIntended UseThis BioAssay™ kit is a sandwich ELISA for in vitro quantitative measurement of Cav-1 in rat serum, plasma and other biological fluidsSensitivity0.094ng/mLRange0.156-10ng/mLSpecificityRatTest PrincipleThis BioAssay™ ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to rat Cav-1 (Caveolin-1). Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for rat Cav-1 (Caveolin-1) and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain rat Cav-1 (Caveolin-1), biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of rat Cav-1 (Caveolin-1). The concentration of rat Cav-1 (Caveolin-1) in samples can be calculated by comparing the OD of the samples to the standard curve.Kit ComponentsMicrotiter Strips, 8x12 wellsStandard, 2 vialsSample/Standard Diluent Buffer, 1x20mlAntibody-Biotin, 1x120ulAntibody-Biotin Diluent, 1x10mlHRP Conjugate, 1x120ulHRP Conjugate Diluent, 1x10mlSubstrate Reagent, 1x10mlStop Solution, 1x10mlWash Buffer (25X), 1x30mlStorage and StabilityUnused kit is stable for 6 months after receipt.  Store Microtiter Strips, Standard, and Antibody-Biotin at 4°C if kit will be used within 30 days; otherwise store at -20°C until expiration date. Store the rest of the components at 4°C. Protect HRP Conjugate and Substrate Reagent from light. For maximum recovery, centrifuge the original vials after thawing and prior to removing the cap.

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