产品说明
Neurotrophin-4(NT-4/NT-5)isapotentneurotrophicfactorwhichsupportsthegrowthandsurvivABIlityofnerveand/orglialcells.NT-4isaneurotrophicfactorstructurallyrelatedtobeta-NGF,BDNFandNT-3.Theseproteinsbelongtothecysteine-knotfamilyofgrowthfactorsthatassumestabledimericstructures.NT-4isexpressedintheprostate,thymus,placentaandskeletalmuscle.NT-4cansignalthroughtheLNGFRandtrkBreceptorsandpromotesthesurvivalofperipheralsensorysympatheticneurons.TheactiveformofhumanNT-4isadimer,formedbytwoidentical130aminoacidsubunits,whichisheldtogetherbystronghydrophobicinteractions.RecombinanthumanNT-4isanoncovalentlylinkedhomodimer,oftwo14.0kDpolypeptidemonomers(260totalaminoacidresidues).||Source:|RecombinantproteincorrespondingtohumanNT-4expressedinE.coli.||BIOLOGicalActivity:|TheED50asdeterminedbythedose-dependentinductionofcholineacetyltransferaseactivityinratbasalforebrainprimaryseptalcellcultureswasfoundintherangeof20-50ng/ml.||Endotoxin:|<0.1ng/ugofprotein(<1EU/ug)||AASequence:|GVSETAPASRRGELAVCDAVSGWVTDRRTAVDLRGREVEV|LGEVPAAGGSPLRQYFFETRCKADNAEEGGPGAGGGGCRG|VDRRHWVSECKAKQSYVRALTADAQGRVGWRWIRIDTACV|CTLLSRTGRA||ProteinContent:|VerifiedbyUVSpectroscopyand/orSDS-PAGEgel.||StorageandStability:|Lyophilizedpowdermaybestoredat-20°C.Stablefor12monthsafterreceiptat-20°C.ReconstitutewithH2O.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Reconstitutedproductisstablefor6monthsat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.
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