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Usbio/Probable Endo-Beta-1,4-Glucanase D, Recombinant, Aspergillus kawachii, aa21-408, His-Tag (eglD)/10ug/518066
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Creatine kinase (CK) is widely distributed and seems to be primarily concerned with ATP regeneration. CK may represent 10-20% of muscle cytoplasmic protein.||Protein:|≥ 0.6mg protein/mg (Coomassie)||Activity:|≥ 250 U/mg protein (Dimension® Clinical Chemistry System)||Specific Activity:|≥ 200U/mg protein||Unit Definition:|One Unit will transfer one micromole of phosphate from Creatine Phosphate to ADP per minute at 37°C. Measured at 340nm as one equimolar amount of NADH produced by a coupled reaction.||Reconstitution:|1mg protein/ml in Tris buffered saline, pH 8.0 containing 1mM dithiothreitol and 1mM EDTA. Add glycerol to 50% (v/v) and store at -20°C. Further dilutions should be in the reconstitution buffer plus 1% BSA as a carrier.||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute andaliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Store dessicated.Source: Rabbit Muscle|Purity: Contaminants: ALKP: < 0.01%, GOT/AST: < 0.01%, GPT: < 0.01%, PK: 0.11%|Form: Supplied as a lyophilized powder.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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