Advancedglycationendproductsinducechangesinglucoseconsumption,lactateproduction,andATPlevelsinSH-SY5Yneuroblastomacellsbyaredox-sensitivemechanism.Advancedglycationendproducts(AGEs)accumulateonlong-livedproteins,includingbeta-amyloidplaquesinAlzheimer'sdisease.Theyaresuggestedtocontributetoneuronaldysfunctionandcelldeath.ArecentstudyatNeuroimmunologicalCellBIOLOGyUnit,Leipzig,GermanyinvestigatedtheeffectsofamodelAGEuponglucosemetabolismandenergyproductioninaneuroblastomacellline.AGEsdecreasecellularATPlevelsandincreaseglucoseconsumptionandlactateproduction.AlloftheAGE-inducedmetabolicchangescanbeattenuatedbyantioxidantssuchas(R+)-alpha-lipoicacidand17beta-estrADIol.Theseantioxidantsmaybecomeusefuldrugsagainst(AGE-mediated)effectsinneurodegenerationthroughtheirpositiveeffectsoncellularenergymetabolism.||Reactionofaminogroupsinproteinwithglucoseleadstotheformationofadvancedglycationendproducts(AGE)throughtheearlyproductssuchasSchiffbaseandAmadorirearrangementproducts.Recentimmunologicalstudiesusinganti-AGEantibodydemonstratedthepresenceofAGE-modifiedproteinsinseveralhumantissues:|(i)humanlens(non-diabeticandnon-cataractous),(ii)renalproximaltubulesinpatientswithdiabeticnephropathyandchronicrenalfailure,(iii)diabeticretina,(iv)peripheralnervesofdiabeticneuropathy,(v)atheroscleroticlesionsofarterialwalls,(vi)b2-microglobulinformingamyloidfibrilsinpatientswithhemodialysis-relatedamyloidosis,(vii)senileplaquesofpatientswithAlzheimerdisease,(viii)peritoneumofCAPDpatients,(ix)skinelastininactinicelastosis,and(x)ceroid/lipofusiondeposits.TheseresultssuggestapotentialroleofAGE-modificationinnormalagingaswellasage-enhanceddiseaseprocesses.||Glycated-BSAshowsa5,000-10,000%increaseinfluorescenceascomparedtonormalBSA(confirmedbyfluorescencespectrophotometry,excitation/emission370/440nm).||StorageandStABIlity:|Aliquottoavoidrepeatedfreezingandthawingandstoreat-70°C.Aliquotsarestablefor6monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap|Note:Duringextendedstorage,AGE-BSAmayprecipitate.Inthiscase,sonicationwillbehelpfultodissolvetheprecipitates.Source:HumanAGEBSAcoupled.PreparedbyreactingBSAwithglycoaldehydeundersterileconditions.|Purity:≥95%bySDS-PAGE|Concentration:~10mg/ml|Form:SuppliedasaliquidinPBS,pH7.2.||ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplicationswithouttheexpressedwrittenauthorizationofUnitedStatesBiological.

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