SampleType:|Serum,plasma,tissuehomogenates,celllysates,cellculturesupernatesandotherBIOLOGicalfluids||IntendedUse:|ThekitisasandwichenzymeimmunoassayforinvitroquantitativemeasurementofMCP1inserum,plasma,tissuehomogenates,celllysates,cellculturesupernatesandotherbiologicalfluids.Duetothe82%homologyofthesequenceamongdifferentspecies,thekitcanbeusedtodetectmouseandratsamples||Sensitivity:|0.064ng/ml||Range:|0.156-10ng/ml||Specificity:|ThisassayhashighsensitivityandexcellentspecificityfordetectionofWide-rangeMonocyteChemotacticProtein1(MCP1).Nosignificantcross-reactivityorinterferencebetweenWide-rangeMonocyteChemotacticProtein1(MCP1)andanalogueswasobserved.||Precision:|Intra-Assay:CV<10%|Inter-Assay:CV<12%||TestPrinciple:|Themicroplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictoMCP1.Standardsorsamplesarethenaddedtotheappropriatemicroplatewellswithabiotin-conjugatedantibodyspecifictoMCP1.Next,AvidinconjugatedtoHorserADIshPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.AfterTMBsubstratesolutionisadded,onlythosewellsthatcontainMCP1,biotin-conjugatedantibodyand|enzyme-conjugatedAvidinwillexhibitachangeincolor.Theenzyme-substratereactionisterminatedbytheadditionofsulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±10nm.TheconcentrationofMCP1inthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.||KitComponents:|96-wellstripplate,1xPlate|Standard,2xvial|DetectionReagentA,1×120ul|DetectionReagentB,1×120ul|TMBSubstrate,1×9ml|WashBuffer(30X),1×20ml|StandardDiluent,1×20ml|AssayDiluentA,1×12ml|AssayDiluentB,1×12ml|StopSolution,1×6ml||StorageandStABIlity:|Storeallcomponentsat4°C.Stablefor6monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplicationswithouttheexpressedwrittenauthorizationofUnitedStatesBiological.

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