Sequence:|5'-CˇC T A G G-3'|3'-G G A T CˆC-5'||Source: |Xanthomonas maltophilia Jo 85–025||Concentration: |10 units/ul||Unit Definition:|One unit is defined as the amount of XmaJI required to digest 1ug of lambda DNA-Smal fragments in 1 hour at 37°C in 50ul of reaction buffer.||Supplied with:|R1625: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.||Diluentand Storage Buffer:|10mM Tris-HCl , pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.Overdigestion Assay:|No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hour) with XmaJI.||Ligation/Recutting Assay:|After 50-fold overdigestion (3u/ug DNA x 17 hours) with XmaJI more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.06uM. More than 95% of these can be recut. ||Labeled Oligonucleotide (LO) Assay:|No detectable degradation of a single-stranded or double-stranded labeled oligonucleotide was observed after incubation with 10 units of XmaJI for 4 hours. ||Recognition Sites:|Lambda: 2|PhiX174: 0|pBR322: 0|pUC57: 0|pUC18/19: 0|pTZ19R/U: 0|M13mp18/19: 0||Blue/White Cloning Assay: |The mix of pUC57/HindIII, pUC57/PstI and pUC57/Eco32I digests was incubated with 10 units of XmaJI for 16 hours. After religation andtransformation <1% of white colonies were detected.||Stability during Prolonged Incubation: |A minimum of 0.2 units of XmaJI is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C. ||Thermal Inactivation: |XmaJI is inactivated by incubation at 80°C for 20 minutes.||Digestion of Agarose-embedded DNA: |A minimum of 10 units of the enzyme is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours. ||Compatible Ends: |BcuI, NheI, XbaI, Eco130I (CˇCTAGG)

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