Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, Mol. Wt. 40-80kD) and Sodium glucose co-transporter-1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. Glut-2 provides glucose to the liver and pancreatic cells. Glut-3 is the main transporter in neurons, whereas Glut-4 (mouse/human 509aa) is primarily expressed in muscle and adipose tissue and regulated by insulin.||Source:|Synthetic peptide corresponding to 13aa from human Glut-4, N-terminal, extracellular domain.||Molecular Weight:|~3kD||Applications: |Suitable for use in ELISA and Antibody Blocking. It is not suitable for use in Western. Other applications not tested.||Recommended Dilution:|ELISA: 5-10ug control peptide per 1ug of aff pure IgG or 1ul antiserum|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.Source: Synthetic peptide|Purity: Purified|Concentration: ~1mg/ml|Form: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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