首页 > Usbio > Usbio/Elastase, Neutrophil, Human, 95+%/100ug/E2230-11

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Usbio/Elastase, Neutrophil, Human, 95+%/100ug/E2230-11

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E2230-11

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Theprimaryroleofneutrophilsistodestroyanyforeignparticles,eitherbyphagocytosisorbyreleaseofbacteriostaticagents.Neutrophilscontainhighconcentrationsoftheserineproteinasesneutrophilelastasethatisstoredinintracellularvacuolestermedtheazurophilicgranules. Thisproteinaseisamajorproteinconstituentofneutrophils,comprisingaround1-3pgperneutrophil. Theirprimaryroleistodigestanypathogensthattheneutrophilhasphagocytosed. |PurulenthumansputumisfractionatedwithsodiumchlorideandtreatedwithDNasetoreleaseDNA-boundproteins.TheelastaseispurifiedbyaffinitychromatographyonAgarose-DAPSA-Elastinandseparatedfromcathepsinsbyionexchangechromatography.Theelastaseisfurtherpurifiedbyaffinitychromatographyonagarose-alanyl-alanyl-alanine.TheelastaseisfreeofcathepsinGactivitywhenassayedwithSuc-AI-AI-Pro-Phe-pNA.||UnitDefinition:|Oneunitwillhydrolyze1umoleofsubstrateperminuteatpH7.5and25°C.||DiluentsandStABIlity:|1.1mg/mlin0.05MNaOAcpH5containing0.1MNaCI.Stableatleast7daysat5°C||2.10mg/mlin50%glycerol50%,0.02MNaOAcpH5.Stableatleast60daysat-20°Cwith20cyclesbetween-20°Cand5°C.||3.Maybedissolvedat10mg/mlin2mMHoAcandre-lyophilizedinsmallerquantities.|Stabilityisbetterinacidic(pH5)thanbasicbuffers.InactivationmayoccuratpH8duetoautolysisand/orproteolysisbytrypsinsuchasbovinepancreatictrypsin.||StorageandStability:|Aliquottoavoidrepeatedfreezingandthawing.Storeat-70°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.Source:Purulenthumansputum|Purity:Purifiedbyaffinitychromatography.≥95%bySDS-PAGE.CathepsinG:AsReported.MPO:AsReported.|Concentration:~1mg/ml|Form:Suppliedasaliquidin0.05Msodiumacetate,0.6Msodiumchloride,pH5.5|Specificity:Activity:≥10unitspermgproteinonthesubstrateMeO-Suc-AI-AI-Pro-Val-pNA.SpecificActivity:||ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplicationswithouttheexpressedwrittenauthorizationofUnitedStatesBIOLOGical.

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