Recombinantproteincorrespondingtoaa219-358withtwoN-TerminalHis-TagandS-tagfrommouseSexHormoneBindingGlobulinexpressedinE.coli(P97497).||AminoAcidSequence:|DVDLQPGLFFPPGTHAEFSLQDIPQPHADPWTFSLELGFKLVDGSGQLLALGTGTNSSWLNIHLQNQSVVLSSEAEPKVVLPLDVGLPLQLTLDRVKVVLSQGPKMEVLSMSLLRPASLWRLWSHPQGHLSLGALPGESS||SubcellularLocation:|Secreted||PredictedMolecularWeight:|20.6kD||PredictedIsoelectricPoint:|5.5||AccurateMolecularWeight:|24kD(SDS-PAGEreducing)|Note:ThepossIBLereasonsthattheactualbandsizediffersfromthepredictedareasfollows:|1.Splicevariants:Alternativesplicingmaycreatedifferentsizedproteinsfromthesamegene|2.Relativecharge:Thecompositionofaminoacidsmayaffectthechargeoftheprotein.|3.Post-translationalmodification:Phosphorylation,glycosylation,methylationetc.|4.Post-translationcleavage:Manyproteinsaresynthesizedaspro-proteinsandthencleavedtogivetheactiveform.|5.Polymerizationofthetargetprotein:Dimerization,multimerizationetc.||Applications:|SuitableforuseinELISA,WesternBlot,ImmunoprecipitationandSDS-PAGE.|Otherapplicationsnottested.||RecommendedDilutions:|Optimaldilutionstobedeterminedbytheresearcher.||IncludedProteinMolecularWeightMarker:|168631:UnstainedProteinMolecularWeightMarker,10-70kD|Suppledasaliquidin62.5mMTris-H3PO4,pH7.5,1mMEDTA,2%SDS,100mMDTT,1mMsodiumazide,0.01%bromo-phenolblue,33%glycerol.Readytouse;noneedtoheat,diluteoraddreducingagentsbeforeuse.||ProteinBands:10kD,14kD,18kD,22kD,26kD,33kD,44kDand70kD.|DoubleIntesityBands:The26kD,18kDand10kDbandsareatdoubleintensitytomakelocationandsizeapproximationofproteinseasier.||Usage:Allowthemarkertoreachroomtemperatureandmixthoroughlybeforeusetoensurethatanysolidsthatmayhaveprecipitatedat-20°Cwillgobackintosolution.Donotboil!LoadthefollowingvolumesonSDS-PAGEgel:|Minigels:3-5ul/well|Largegel:7ul/well|TheloADIngvolumeslistedabovearerecommendedforgelswiththicknessof0.75-1mm.Theloadingvolumeshouldbedoubledfor1.5mmthickgels.|Storeat-20°CStablefor6monthsafterreceipt.||StorageandStABIlity:|Lyophilizedpowdermaybestoredat-70°C.Stablefor6monthsafterreceipt.ReconstitutewithsterileddH2O.Aliquottoavoidrepeatedfreezingandthawing.Storeat-70°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.||Note:|Thermalstabilityisdescribedbythelossrateofthetargetprotein.Thelossratewasdeterminedbyacceleratedthermaldegradationbyincubatingtheproteinat37°Cfor48h,withnoobviousdegradationorprecipitationobserved.Proteinlossis<5%withintheexpirationdateunderappropriatestorageconditions.Source:E.coli|Purity:≥95%Endotoxin(LAL):≤1EU/ug|Form:Suppliedaslyophilizedpowderin10mMPBS,pH7.4,5%trehalose0.01%sarcosyl,0.02%sodiumazide.ReconstituteinsterileddH2O.||ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplicationswithouttheexpressedwrittenauthorizationofUnitedStatesBIOLOGical.

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