产品说明
TSEN15 (tRNA-splicing endonuclease subunit Sen15) belongs to the SEN15 family. tRNA splicing is a fundamental process required for cell growth and division. SEN15 is a subunit of the tRNA splicing endonuclease. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron.|Purified by using conventional chromatography techniques.||Source:|Recombinant human corresponding to aa1-171 of TSEN15, fused to His-tag at N-terminus, expressed in E.coli.||AA Sequence:|MGSSHHHHHH SSGLVPRGSH MEERGDSEPT PGCSGLGPDG VRGFGDGGGA PSWAPEDAWM GTHPKYLEMM ELDIGDATHV YVAFLVYLDL MESKSWHEVN CVGLPELQLI CLVGTEIEGE GLQTVVPTPI TASLSHNRIR EILKASRKLQ GDPDLPMSFT LAIVESDSTI VYYKLTDGFM|LPDPQNISLR R||Molecular Weight: |~20.9kDa (191aa) confirmed by MALDI-TOF||Enzyme Activity:|Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.Source: E.coli|Purity: ~90% (SDS-PAGE)|Concentration: ~0.5mg/ml|Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8, 2mM DTT, 200mM sodium chloride, 20% glycerol.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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