In1990anoncogene,v-mpl,wasidentifiedfromthemurinemyeloproliferativeleukemiavirusthatwascapableofimmortalizingbonemarrowhematopoieticcellsfromdifferentlineages.In1992thehumanhomologue,named,c-mpl,wascloned.Sequencedatarevealedthatc-mplencodedaproteinthatwashomologouswithmembersofthehematopoieticreceptorsuperfamily.Presenceofanti-senseoligodeoxynucleotidesofc-mplinhibitedmegakaryocytecolonyformation.Theligandforc-mpl,thrombopoietin,wasclonedin1994.Thrombopoietinwasshowntobethemajorregulatorofmegakaryocytopoiesisandplateletformation.Theproteinencodedbythec-mplgene,CD110,isa635aatransmembranedomain,withtwoextracellularcytokinereceptordomainsandtwointracellularcytokinereceptorboxmotifs.TPO-Rdeficientmicewereseverelythrombocytopenic,emphasizingtheimportantroleofCD110andthrombopoietininmegakaryocyteandplateletformation.UponbindingofthrombopoietinCD110isdimerizedandtheJAKfamilyofnon-receptortyrosinekinases,aswellastheSTATfamily,theMAPKfamily,theadaptorproteinShcandthereceptorsthemselvesbecometyrosinephosphorylated.||Applications:|SuitableforuseinWesternBlot,ELISAandFlowCytometry.Otherapplicationsnottested.||RecommendedDilution:|ELISA:1:10,000|WesternBlot:1:500-1:2000,incubatemembranewithdilutedprimaryantibodyinTBS,5%milk,0.1%Tween20at4°Cwithgentleshaking,overnight.|FlowCytometry:1:200-1:400|ELISA(peptide):1:20,000-1:40,000|Optimaldilutionstobedeterminedbytheresearcher.||StorageandStABIlity:|Maybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestablefor12monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.ProductType:Mab|Isotype:IgG1|Host:Mouse|Source:human|Concentration:~1mg/ml|Form:SuppliedasaliquidinPBS(withoutMg2+andCa2+),pH7.4,150mMsodiumchloride,0.02%sodiumazide,50%glycerol.|Purity:Purifiedbyaffinitychromatography.|Immunogen:PurifiedrecombinantfragmentcorrespondingtohumanMPLexpressedinE.coli.|Specificity:RecognizesendogenouslevelsoftotalhumanMPL.||ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplicationswithouttheexpressedwrittenauthorizationofUnitedStatesBIOLOGical.

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