首页 > Usbio > Usbio/C2069-43-AP Mouse Anti-Catenin Alpha1 (CTNNA1, Catenin (Cadherin-associated Protein), Alpha 1, 102kDa, HGNC:2509, CAP102, FLJ36832, Alpha 1 (102kD), Alpha-E-Catenin, Alpha-Catenin, AlphaE-Catenin) (AP)/C2069-43-AP/100ul

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Usbio/C2069-43-AP Mouse Anti-Catenin Alpha1 (CTNNA1, Catenin (Cadherin-associated Protein), Alpha 1, 102kDa, HGNC:2509, CAP102, FLJ36832, Alpha 1 (102kD), Alpha-E-Catenin, Alpha-Catenin, AlphaE-Catenin) (AP)/C2069-43-AP/100ul

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a-cateninisa102kDproteinwhichisrelatedtothecytoskeletalproteinvinculin.Vinculinassociateswithbothcell-cellandcell-extracellularmatrixadherens-typejunctions,andhasbeenshowntointeractwithbotha-actininandactin.Thehomologybetweena-cateninandvinculinislocalizedtothreemajorregions(amino-terminal,central,andcarboxy-terminalportions)withthec-terminiexhibitingthehighestoverallhomology.Interestingly,itisthecarboxyterminaldomainofvinculinwhichisresponsIBLeforitsself-associationproperties.Thehomologybetweenthec-terminiofcateninandvinculinledtospeculationthata-catenincanself-associateand/orco-associatewithvinculintherebylinkingthecadherin/catenincomplextotheCytoskeleton.However,recentstudiesindicatethatcadherin/catenincomplexesarelinkedtotheactincytoskeletonviaadirectassociationbetweena-actininanda-catenin.Inaddition,thea-cateninproteinhasbeenfoundtopossesbothactinbindingandbundlingactivities.Applications:|SuitableforuseinImmunofluorescence,ELISA,WesternBlot,ImmunoprecipitationandImmunohistochemistry.Otherapplicationsnottested.RecommendedDilutionImmunofluorescence:2-5ug/mlELISA:0.1-1ug/mlWesternBlot:1ug/mlImmunohistochemistry(formalin-ﬁxed,parafﬁn-embeddedtissue):2-10ug/ml.Heat-InducedEpitopeRetrieval(HIER)isrequired.Expectedstainingpattern:Membranewithlimitedcytoplasmic.Immunoprecipitation:5ugOptimaldilutionstobedeterminedbytheresearcher.PositiveControl:|Pancreas/breasttumor/heartSource:|AscitesStorageandStABIlityMaybestoredat4°Cforshort-termonly.Forlong-termstorage,storeat-20°C.Aliquotsarestableforatleast12monthsat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.Note:Applicationsarebasedonunconjugatedantibody.ImmunogenSyntheticpeptidederivedfromtheC-terminusofmousea-catenin.FormSuppliedasaliquidinPBS,pH7.2.Nopreservativesadded.LabeledwithAlkalinePhosphatase(AP).PurityPurifiedbyimmunoaffinitychromatography.SpecificityRecognizesmousea-catenin.Doesnotcrossreactwiththerelatedbeta-andgamma-cateninproteins.SpeciesCrossreactivity:Human,rat,chicken,Xenopus.LysatesTested:HeLacells,A431cells,WI-38humanﬁbroblasts,Xenopuscytosol,NIH3T3cells.

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