Tightjunctionsarespecializedregionsofcell-cellcontactthatareparticularlyabundantinluminalepithelialcellsheets.Infreeze-fractureelectronmicrographs,tightjunctionsarevisualizedasbelt-likebandsofanastomosingsealingstrands|(TJstrands)thatcompletelyencirclethelateralsurfacesofeachcell.TJstrandsonadjacentcellsarepresumedtointeractwitheachothertoformasortof“moleculargasket”thatpreventsions,waterandothermoleculesfromleakingbetweencellsandthus,fromonesideofthesheettotheother.Inadditiontothisso-called“barrier”function,the“fence”functionoftightjunctionsplaysanimportantroleinmaintainingepithelialcell-polaritybyblockingthediffusionofmembraneproteinsbetweenapical(luminal)andbasolateralcellsurfaces.|Claudin-7islocalizedintheintercellularjunctionsoftheepithelialcellsoftheproximaltubulesofthenephronsinmammaliankidney1.Itisreportedlydownregulatedinheadandnecksquamouscellcarcinomas2.Claudin-7isalsoexpressedinnormalhumanandmouseepithelia.Applications:|SuitableforuseinImmunofluorescence,WesternBlotandImmunohistochemistry.Otherapplicationsnottested.RecommendedDilutionImmunofluorescence(Canine):1-2ug/mlWesternBlot:1-2ug/mlImmunohistochemistry(Human,mouse):1-2ug/ml.Priortostaining,formalin-fixed,paraffin-embeddedtissuesrequiresheat-inducedepitoperetrieval(HIER)withcitratebuffer,pH6.Optimaldilutionstobedeterminedbytheresearcher.StorageandStABIlityMaybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestableforatleast12months.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap. Note:Applicationsarebasedonunconjugatedantibody.ImmunogenSyntheticpeptidederivedfromtheC-terminalregionofthehumanClaudin-7protein.CellularLocalization:CytoplasmicFormSuppliedasaliquidinPBS,pH7.2.Nopreservativesadded.LabeledwithAlkalinePhosphatase(AP).PurityPurifiedbyimmunoaffinitychromatography.SpecificityReactswiththehumanClaudin-7.ReactivityisconfirmedwithhumanT47Dcelllysates,humanandmousebreasttissuesandcanineMDCKcelllysates.References1.ReyesJL,etal.KidneyInt62:476-487,20022.AlMoustafaAE,etal.Oncogene21:2634-2640,2002.

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