首页 > Usbio > 303194-HRP Rabbit Anti-CCL3 (C C Motif Chemokine 3, CCL 3, CCL3_HUMAN, Chemokine C C Motif Ligand 3, Chemokine CC Motif Ligand 3, G0/G1 Switch Regulatory Protein 19 1, G0/G1 Switch Regulatory Protein 19-1, G0S19 1, G0S19 1 Protein, Heparin Binding Chemota

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303194-HRP Rabbit Anti-CCL3 (C C Motif Chemokine 3, CCL 3, CCL3_HUMAN, Chemokine C C Motif Ligand 3, Chemokine CC Motif Ligand 3, G0/G1 Switch Regulatory Protein 19 1, G0/G1 Switch Regulatory Protein 19-1, G0S19 1, G0S19 1 Protein, Heparin Binding Chemota

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Macrophageinflammatoryprotein-1alpha(MIP-1alpha),alsocalledCCL3,LD78.TheCDNAforCCL3predictsamaturepeptideof69aawithamolecularmassof7,889daltons.CCL3isamemberofanewlyidentifiedsuperfamilyofsmallinducIBLeproteinsinvolvedininflammatoryresponses,woundhealingandtumOrigenesis.CCL3isachemokinethathaspro-inflammatoryandstemcellinhibitoryactivitiesinvitro.Itconstitutesanimportantsecondsignalformastcelldegranulationintheconjunctivainvivoandconsequentlyforacute-phasedisease.Applications:|SuitableforuseinELISAandWesternBlot.Otherapplicationsnottested.RecommendedDilutionELISA:0.1-0.5ug/mlWesternBlot:0.1-0.5ug/ml,thedetectionlimitis~1ng/laneunderreducingconditions.Optimaldilutionstobedeterminedbytheresearcher.StorageandStABIlityLyophilizedpowdermaybestoredat-20°C.Stablefor12monthsafterreceiptat-20°C.ReconstitutewithsterileddH2O.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Reconstitutedproductisstablefor12monthsat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.Note:Applicationsarebasedonunconjugatedantibody.ImmunogenRecombinantproteincorrespondingtoratCCL3expressedinE.coli.(Position:A24-A92).Speciessequencehomology:human(74%)andmouse(91%)FormSuppliedasaliquidinPBS,pH7.2.LabeledwithhorserADIshperoxidase(HRP).PurityPurifiedbyimmunoaffinitychromatography.SpecificityRecognizesratCCL3.References1.Wolpe,S.D.;Cerami,A.Macrophageinflammatoryproteins1and2:membersofanovelsuperfamilyofcytokines.FASEBJ.3:2565-2573,1989.2.Nakao,M.;Nomiyama,H.;Shimada,K.StructuresofhumangenescodingforcytokineLD78andtheirexpression.Molec.Cell.Biol.10:3646-3658,1990.3.Cook,D.N.;Beck,M.A.;Coffman,T.M.;Kirby,S.L.;Sheridan,J.F.;Pragnell,I.B.;Smithies,O.RequirementofMIP-1-alphaforaninflammatoryresponsetoviralinfection.Science269:1583-1585,1995.4.Miyazaki,D.;Nakamura,T.;Toda,M.;Cheung-Chau,K.-W.;Richardson,R.M.;Ono,S.J.Macrophageinflammatoryprotein-1-alphaasacostimulatorysignalformastcell-mediatedimmediatehypersensitivityreactions.J.Clin.Invest.115:434-442,2005.

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