首页 > Usbio > 303592-HRP Rabbit Anti-Serum Amyloid P (9.5S Alpha 1 Glycoprotein, 9.5S Alpha-1-glycoprotein, Amyloid P Component Serum, APCS, Pentaxin Related, Pentraxin 2, PTX 2, PTX2, SAMP_HUMAN, SAP, Serum Amyloid P Component, Serum Amyloid P-component(1-203)) (HRP)

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303592-HRP Rabbit Anti-Serum Amyloid P (9.5S Alpha 1 Glycoprotein, 9.5S Alpha-1-glycoprotein, Amyloid P Component Serum, APCS, Pentaxin Related, Pentraxin 2, PTX 2, PTX2, SAMP_HUMAN, SAP, Serum Amyloid P Component, Serum Amyloid P-component(1-203)) (HRP)

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AmyloidPcomponent,serum(SAP),alsoknownasPTX2orAPCS,istheidenticalserumformofamyloidPcomponent(AP),a25kDpentamericproteinfirstidentifiedasthepentagonalconstituentofinvivopathologicaldepositscalled"amyloid".Itbelongstothepentraxinsfamily,characterisedbycalciumdependentligandbindinganddistinctiveflattenedbeta-jellyrollstructuresimilartothatofthelegumelectins.Thisgeneismappedto1q23.2.Thebindingoftheencodedproteintoproteinsinthepathologicalamyloidcross-betafoldsuggestsitspossIBLeroleasachaperone.Thisproteinisalsothoughttocontrolthedegradationofchromatin.Ithasbeendemonstratedthatthisproteinbindstoapoptoticcellsatanearlystage,whichraisesthepossibilitythatitisinvolvedindealingwithapoptoticcellsinvivo.Applications:|SuitableforuseinWesternBlotandImmunohistochemistry.Otherapplicationsnottested.RecommendedDilutionWesternBlot:0.1-0.5ug/mlImmunohistochemistry(paraffin):0.5-1ug/ml,boilingtheparaffinsectionsin10mMcitratebuffer,pH6.0,for20minsisrequiredforthestainingofformalin/paraffinsections.Optimaldilutionstobedeterminedbytheresearcher.StorageandStABIlityLyophilizedpowdermaybestoredat-20°C.Stablefor12monthsafterreceiptat-20°C.ReconstitutewithsterileddH2O.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Reconstitutedproductisstablefor12monthsat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.Note:Applicationsarebasedonunconjugatedantibody.ImmunogenRecombinantproteincorrespondingtoratSerumAmyloidPexpressedinE.coli.(Position:Q21-S228).Speciessequencehomology:human(72%)andmouse(80%)FormSuppliedasaliquidinPBS,pH7.2.LabeledwithhorserADIshperoxidase(HRP).PurityPurifiedbyimmunoaffinitychromatography.SpecificityRecognizesratSerumAmyloidP.References1.CathcartES,ShirahamaT,CohenAS(1967)."Isolationandidentificationofaplasmacomponentofamyloid".Biochim.Biophy.Acta147:392–393.2."EntrezGene:APCSamyloidPcomponent,serum".3.EmsleyJ,WhiteHE,O'HaraBP,OlivaG,SrinivasanN,TickleIJ,BlundellTL,PepysMB,WoodSP(1994)."StructureofpentamerichumanserumamyloidPcomponent".Nature367(6461):338–45.

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