Cullin-3(Cul3),isaproteinthatinhumansisencodedbytheCUL3gene.Kipreosetal.(1996)identifiedaconservedgenefamily,designatedcullins,withatleast5membersinnematodes,6inhumans,and3inS.cerevisiae.HumanCUL3isanorthologofnematodecul3.Hartz(2012)mappedtheCUL3genetochromosome2q36.2basedonanalignmentoftheCUL3sequencewiththegenomicsequence(GRCh37).Sumaraetal.(2007)foundthatKLHL9,KLHL13,andCUL3interacteddirectlyina370-kDproteincomplexinHeLacelllysates.Rondouetal.(2008)showedthatinteractionbetweenKLHL12andtheCUL3ubiquitinligasecomplexdirectedubiquitinationofdopaminereceptorD4(DRD4).KLHL12interacteddirectlywithCUL3andwiththepolymorphicintracellularloop-3ofD4.ByimmunoprecipitationanalysisofHeLacellsandHCT116humancoloncarcinomacellsexpressingepitope-taggedproteins,Kigoshietal.(2011)showedthatKLHL7interactedwithCUL3andROC1(RBX1).UniProtNumberQ13618GeneIDCUL3ApplicationsSuitableforuseinWesternBlot.Otherapplicationsnottested.RecommendedDilutionOptimaldilutionstobedeterminedbytheresearcher.StorageandStABIlityStoreproductat4°Cinthedark.DONOTFREEZE!Stableat4°Cfor12monthsafterreceiptasanundilutedliquid.Diluterequiredamountonlypriortoimmediateuse.Furtherdilutionscanbemadeinassaybuffer.Caution:PEconjugatesaresensitivetolight.Formaximumrecoveryofproduct,centrifugetheoriginalvialpriortoremovingthecap.Note:Applicationsarebasedonunconjugatedantibody.ImmunogenAsyntheticpeptidecorrespondingtoaa426-444,DVFERYYKQHLARRLLTNKinthemiddleregionofhumanCullin3(Q13618).SpeciesSequenceHomology:mouseFormSuppliedasaliquidinPBS,pH7.2.LabeledwithR-Phycoerythrin(PE).PurityPurifiedbyimmunoaffinitychromatography.SpecificityRecognizeshumanCullin3.SpeciesCrossreactivity:rat.Nocrossreactivitywithotherproteins.References1.Kigoshi,Y.,Tsuruta,F.,Chiba,T.UbiquitinligaseactivityofCul3-KLHL7proteinisattenuatedbyautosomaldominantretinitispigmentosacausativemutation.J.Biol.Chem.286:33613-33621,2011.2.Kipreos,E.T.,Lander,L.E.,Wing,J.P.,He,W.W.,Hedgecock,E.M.cul-1isrequiredforcellcycleexitinC.elegansandidentifiesanovelgenefamily.Cell85:829-839,1996.3.Rondou,P.,Haegeman,G.,Vanhoenacker,P.,VanCraenenbroeck,K.BTBproteinKLHL12targetsthedopamineD4receptorforubiquitinationbyaCul3-basedE3ligase.J.Biol.Chem.283:11083-11096,2008.4.Sumara,I.,Quadroni,M.,Frei,C.,Olma,M.H.,Sumara,G.,Ricci,R.,Peter,M.ACul3-basedE3ligaseremovesAuroraBfrommitoticchromosomes,regulatingmitoticprogressionandcompletionofcytokinesisinhumancells.Dev.Cell12:887-900,2007

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