首页 > Usbio > Usbio/E2230-13G-Biotin Mouse Anti-Elastase, Neutrophil (ELANE, ELA2, GE, HLE, HNE, NE, PMN-E, SCN1) (Biotin)/E2230-13G-Biotin/100ul

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Usbio/E2230-13G-Biotin Mouse Anti-Elastase, Neutrophil (ELANE, ELA2, GE, HLE, HNE, NE, PMN-E, SCN1) (Biotin)/E2230-13G-Biotin/100ul

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E2230-13G-Biotin

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Theprimaryroleofneutrophilsistodestroyanyforeignparticles,eitherbyphagocytosisorbyreleaseofbacteriostaticagents.Neutrophilscontainhighconcentrationsoftheserineproteinasesneutrophilelastasethatisstoredinintracellularvacuolestermedtheazurophilicgranules. Thisproteinaseisamajorproteinconstituentofneutrophils,comprisingaround1-3pgperneutrophil. Theirprimaryroleistodigestanypathogensthattheneutrophilhasphagocytosed. Applications:|SuitableforuseinELISA,FlowCytometryandFunctionalAssays.Otherapplicationsnottested.RecommendedDilutionsOptimaldilutionstobedeterminedbytheresearcher.RecommendedSecondaryAntibodiesI1904-06F:IgG,H&L(FITC)(X-Adsorbed)PabGtxMoI1904-23:IgG,X-Adsorbed(DTAF)PabGtxMoI1904-06C:IgG,H&L(HRP)(X-Adsorbed)PabGtxMoI1904-06H:IgG,H&L(HRP)(X-Adsorbed)PabGtxMoI1904-06D:IgG,H&L,X-Adsorbed(AP)PabGtxMoI1904-06J:IgG,H&L,X-Adsorbed(AP)PabGtxMoStorageandStABIlityMaybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestablefor12monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Note:Applicationsarebasedonunconjugatedantibody.ImmunogenElastasefromhumanneutrophils.FormSuppliedasaliquidinPBS,pH7.2.LabeledwithBiotin.PurityPurifiedbyProteinGaffinitychromatography.SpecificityRecognizeshumanneutrophilelastase.References1.Durant,S.etal.(2004)Apoptosis-inducedproteinase3membraneexpressionisindependentfromdegranulation.J.Leukoc.Biol.75(1):87-98.2.Davies,P.L.etal.(2008)Monoclonalanti-neutrophilelastaseantibodycharacterisation:abilitytoblockfunction,detectfreeversusserpin-complexedenzymeandstainintracellulargranules.JImmunolMethods.336:175-82.

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