CD163, a 120kD cell surface glycoprotein is expressed on cells of the monocyte/macrophage lineage. The expression levels of CD163 vary during the course of macrophage differentiation. The highest levels of CD163 expression are found on tissue macrophages but bone marrow derived cells are CD163 negative. |Reported to inhibit both African swine fever infection and viral particle binding to alveolar macrophages in a dose-dependent manner. ApplicationsSuitable for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Westen Blot. Other applications not tested. Recommended DilutionsFlow Cytometry: 1:50-1:200; 10ul labels 10e6 cells in 100ulImmunohistochemistry: Frozen sectionsWestern Blot: Non-reducing conditions Optimal dilutions to be determined by the researcher. Hybridoma: |X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.Recommended Secondary AntibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMoI1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMoStorage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Note: Applications are based on unconjugated antibody.ImmunogenPorcine alveolar macrophagesFormSupplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).PurityPurified by Protein A affinity chromatography.SpecificityRecognizes porcine CD163 at ~120kD in alveolar macrophage cell lysates under non-reducing conditions.References1. Sanchez, M. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation. J. Immunol. 162: 5230-5237. 2. Bullido, R. et al. (1997) Monoclonal antibodies specific for porcine monocyte/ macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens. Tissue Antigens 49: 403-413. 3. Yang, P. et al. (2002) Immune cells in the porcine retina: Distribution, characterization and morphological features.|Invest. Opthalmol. Vis. Sci. 43: 1488-1492. 4. Gomez del Moral, M. et al. (1999) African swine fever virus infection induces tumor necrosis factor alpha production: implications in pathogenesis J. Virol. 73: 2173-2180. 5. Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers. Vet. Immunol. Immunopathol. 80: 93-109. 6. Sanchez-Torres, C. et al. (2003) Expression of porcine CD163 on monocytes/ macrophages correlates with permissiveness to African swine fever infection. Arch. Virol. 148: 2307-2323. 7. Delrue, I. et al. (2010) Susceptible cell lines for the production of porcine reproductive and respiratory syndrome virus by stable transfection of sialoadhesin and CD163. BMC Biotechnol. 10: 48. 8. Katchman, H. et al. (2008) Embryonic porcine liver as a source for transplantation: advantage of intact liver implants over isolated hepatoblasts in overcoming homeostatic inhibition by the quiescent host liver. Stem Cells. 26: 1347-55. 9. Moreno, S.et al. (2010) Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2. Vet Res. 41: 76. 10. Ondrackova, P. et al. (2010) Porcine mononuclear phagocyte subpopulations in the lung, blood and bone marrow: dynamics during inflammation induced by Actinobacillus pleuropneumoniae. Vet Res. 41: 64. 11. Urbieta Caceres, V.H. et al. (2011) Early experimental hypertension preserves the myocardial microvasculature but aggravates cardiac injury distal to chronic coronary artery obstruction. Am J Physiol Heart Circ Physiol. 300: H693-701. 12. Das, P.B. et al. (2010) The minor envelope glycoproteins GP2a and GP4 of porcine reproductive and respiratory syndrome virus interact with the receptor CD163. J Virol. 84: 1731-40. 13. Gimeno, M. et al. (2011) Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.

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