首页 > Usbio > 138219-Biotin Mouse Anti-PHD2 (PH2, Prolyl Hydroxylase Domain Containing Protein 2, HIF2PH2, HIF-Prolyl Hydroxylase 2, EGLN1, Egl Nine Homolog 1, C1orf12, Chromosome 1 Open Reading Frame 12, PNAS-118, PNAS-137, Hypoxia-inducible Factor Prolyl Hydroxylase

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138219-Biotin Mouse Anti-PHD2 (PH2, Prolyl Hydroxylase Domain Containing Protein 2, HIF2PH2, HIF-Prolyl Hydroxylase 2, EGLN1, Egl Nine Homolog 1, C1orf12, Chromosome 1 Open Reading Frame 12, PNAS-118, PNAS-137, Hypoxia-inducible Factor Prolyl Hydroxylase

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PHD1 (Egln2), PHD-2 (Egln1), and PHD3 (Egln3) are members of the Egln family of proline hydroxylases. The EGLN family, which are known to be cellular oxygen sensors, initiates the first step of HIF degradation through the VHL/ubiquitin pathway. PHD1 and PHD2 can hydroxylate both proline 402 and proline 564, but PHD3 can only hydroxylate proline 564. In addition to HIF, PHD enzymes have also been shown to catalyze the hydroxylation of RNA polymerase subunits and myogenin.ApplicationsSuitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.Recommended DilutionImmunofluorescence: 1:500-1:1000Optimal dilutions to be determined by the researcher.HybridomaF0 myeloma cells with spleen cells from Balb/c mice.Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Note: Applications are based on unconjugated antibody.ImmunogenRecombinant protein corresponding to aa271-426 from EGLN1, expressed in E.coli.FormSupplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.PurityPurified by Protein G affinity chromatography.SpecificityRecognizes human EGLN1.

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