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Usbio/G8235-52F-ML750 Rabbit Anti-GPC4, NT (Glypican-4, K-glypican, Secreted Glypican-4, UNQ474/PRO937) (MaxLight 750)/G8235-52F-ML750/100ul
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MaxLight™750isanewNearIRstabledyeconjugatecomparabletoDyLight™750,AlexaFluor™700andoffersbetterlabelingefficiency,brighterimagingandincreasedimmunodetection.Absorbance(759nm);Emission(780nm);ExtinctionCoefficient240,000.Cellsurfaceheparansulfateproteoglycansarecomposedofamembrane-associatedproteincoresubstitutedwithavariablenumberofheparansulfatechains.Membersoftheglypican-relatedintegralmembraneproteoglycanfamily(GRIPS)containacoreproteinanchoredtothecytoplasmicmembraneviaaglycosylphosphatidylinositollinkage.Theseproteinsmayplayaroleinthecontrolofcelldivisionandgrowthregulation.TheGPC4geneisadjacenttothe3'endofGPC3andmayalsoplayaroleinSimpson-GolABI-Behmelsyndrome.Applications:|SuitableforuseinFLISAandImmunohistochemistry.Otherapplicationsnottested.RecommendedDilutionFLISA:1:1,000Immunohistochemistry:1:10-1:50Optimaldilutionstobedeterminedbytheresearcher.StorageandStabilityMaybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestableforatleast12months.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Note:Applicationsarebasedonunconjugatedantibody.ImmunogenSyntheticpeptideselectedfromtheN-termregionofhumanGPC4(KLH).FormSuppliedasaliquidinPBS,pH7.2.Nopreservativeadded.LabeledwithMaxLight™750.PurityPurifiedbyammoniumsulfateprecipitation.SpecificityRecognizeshumanGPC4.References1.Karumanchi,S.A.,Mol.Cell7(4),811-822(2001).2.Hagihara,K.,Dev.Dyn.219(3),353-367(2000).3.Veugelers,M.,Hum.Mol.Genet.9(9),1321-1328(2000).4.Veugelers,M.,Genomics53(1),1-11(1998).

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