首页 > Usbio > Usbio/N2175-06-HRP Mouse Anti-Neuron Specific Enolase, Gamma (gNSE) (HRP)/N2175-06-HRP/100ul

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Usbio/N2175-06-HRP Mouse Anti-Neuron Specific Enolase, Gamma (gNSE) (HRP)/N2175-06-HRP/100ul

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N2175-06-HRP

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Enolases are homo- or heterodimers of the three subunits: alpha (46kD), beta (44kD) and gamma (46kD). The alpha-subunit is expressed in most tissues. The beta-subunit is expressed only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.Applications: |Suitable for use in ELISA, ImmunoBlot. Other applications not tested.Recommended DilutionOptimal dilutions to be determined by the researcher.Matched PairsCapture: N2175-07, N2175-04, N2175-09, N2175-06Detection: N2175-06, N2175-07Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.Note: Applications are based on unconjugated antibody.ImmunogenPurified neuron-specific enolase from human brain.FormSupplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with horseradish peroxidase (HRP).PurityProtein A Sepharose chromatography. Purity is tested by electrophoresis.SpecificityGamma-enolase. Specific for the gamma subunit without detectable crossreactivity with the alpha or beta subunits. Reacts with 45kD band corresponding to NSE in immunoblotting of human brain extracts.

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