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Usbio/502209 Rabbit Anti-PHC3, CT/502209/200ul

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Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.Applications: |Suitable for use in ELISA, Western Blot, Immunohistochemistry. Other applications not tested.Recommended DilutionWestern Blot: 1:2000Immunohistochemistry (Paraffin): 1:25Optimal dilutions to be determined by the researcher.Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.ImmunogenSynthetic peptide corresponding to aa819-850 of human PHC3, KLH conjugated.FormSupplied as a liquid in PBS, 0.09% sodium azide.PurityPurified by Protein A and peptide affinity chromatography.SpecificityRecognizes human PHC3.References1. Tonkin E.,et al.Hum. Genet. 111:435-442(2002).|2. Levine S.S.,et al.Mol. Cell. Biol. 22:6070-6078(2002).|3. Hansen M.F.,et al.Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.|4. Ota T.,et al.Nat. Genet. 36:40-45(2004).|5. Bechtel S.,et al.BMC Genomics 8:399-399(2007).

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