MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.CALD1 is a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction.Applications: |Suitable for use in FLISA, Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.Recommended DilutionFLISA: 1:1000Flow Cytometry: 1:10-1:50Immunohistochemistry: 1:10-1:50Western Blot: 1:50-1:100Optimal dilutions to be determined by the researcher.Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Note: Applications are based on unconjugated antibody.ImmunogenKLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa435-465 from the center region of human CALD1 (Accession #Q05682).FormSupplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.PurityPurified by ammonium sulfate precipitation.SpecificityRecognizes human CALD1.References1. Yoshio,T., FEBS Lett. 581(20), 3777-3782 (2007). 2. Mani,R.S., Biochemistry 31(47), 11896-11901 (1992).

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