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033519-ML550 Rabbit Anti-CD49e, CT (ITGA5, FNRA, Integrin Alpha-5, CD49 Antigen-like Family Member E, Fibronectin Receptor Subunit Alpha, Integrin Alpha-F, VLA-5, CD49e, Integrin Alpha-5 Heavy Chain, Integrin Alpha-5 Light Chain) (MaxLight 550)
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MaxLight™ 550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.CD49e belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein is the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.ApplicationsSuitable for use in Western Blot, Flow Cytometry, FLISARecommended DilutionFLISA: 1:1,000Western Blot: 1:50-100Flow Cytometry: 1:10-50Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Note: Applications are based on unconjugated antibody.ImmunogenCD49e antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 803~832 amino acids from the C-terminal region of human CD49e.FormSupplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.PurityPurified by saturated ammonium sulfate precipitation followed by dialysis against PBS.SpecificityHuman, mouseReferencesBoehmler,A.M., J. Immunol. 182 (11), 6789-6798 (2009)|Okazaki,T., Am. J. Pathol. 174 (6), 2378-2387 (2009)|Schornberg,K.L., Proc. Natl. Acad. Sci. U.S.A. 106 (19), 8003-8008 (2009)

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